Phospho-Synapsin I (Ser605) Antibody - #AF2416
(1)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2416, RRID:AB_2845430.
展开/折叠
Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI;
抗原和靶标
- P17600 SYN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAGRPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17600 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Q14012 (CAMK1) , Q13131 (PRKAA1) | Uniprot |
| S11 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S67 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S293 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
| T448 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | P27361 (MAPK3) , Q00535 (CDK5) | Uniprot |
| S553 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| T567 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| T613 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot |
研究背景
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.
Cell junction>Synapse. Golgi apparatus.
Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP (By similarity).
The A region binds phospholipids with a preference for negatively charged species.
Belongs to the synapsin family.
文献引用
Application: WB Species: Mice Sample: cortex brain tissue
Application: WB Species: mouse Sample: N2a cells
限制条款
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