Phospho-TLK1 (Ser743) Antibody - #AF2425

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产品: 磷酸化 TLK1 (Ser743) 抗体
货号: AF2425
描述: Rabbit polyclonal antibody to Phospho-TLK1 (Ser743)
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 86kDa; 87kD(Calculated).
蛋白号: Q9UKI8
RRID: AB_2845438

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-TLK1 (Ser743) Antibody detects endogenous levels of TLK1 only when phosphorylated at Ser743.
RRID:
AB_2845438
引用格式: Affinity Biosciences Cat# AF2425, RRID:AB_2845438.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

PKU beta; PKU-beta; serine threonine protein kinase; Serine/threonine protein kinase tousled like 1; Serine/threonine-protein kinase tousled-like 1; SNARE protein kinase SNAK; Tlk1; TLK1_HUMAN; Tousled like kinase 1; Tousled-like kinase 1;

抗原和靶标

免疫原:
Uniprot:

Q9UKI8(TLK1_HUMAN) >>Visit HPA database.

基因/基因ID:
TLK1(9874)
表达:
Q9UKI8 TLK1_HUMAN:

Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

序列:
MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UKI8 作为底物

Site PTM Type Enzyme
S22 Phosphorylation
S33 Phosphorylation
T38 Phosphorylation
S41 Phosphorylation
S54 Phosphorylation
T68 Phosphorylation
S72 Phosphorylation
S74 Phosphorylation
S77 Phosphorylation
T78 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
T89 Phosphorylation
S93 Phosphorylation
S94 Phosphorylation
S97 Phosphorylation
S100 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
S133 Phosphorylation
S134 Phosphorylation
S138 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
S170 Phosphorylation
S176 Phosphorylation
S188 Phosphorylation
T192 Phosphorylation
K234 Acetylation
T304 Phosphorylation
T313 Phosphorylation
S357 Phosphorylation
K412 Ubiquitination
S444 Phosphorylation
K485 Ubiquitination
K513 Ubiquitination
S563 Phosphorylation
S611 Phosphorylation
S679 Phosphorylation
T688 Phosphorylation
K700 Ubiquitination
S741 Phosphorylation
S743 Phosphorylation O14757 (CHEK1)
S744 Phosphorylation
S760 Phosphorylation

翻译修饰 - Q9UKI8 作为激酶

Substrate Site Source
Q99638 (RAD9A) S328 Uniprot
Q99638 (RAD9A) T355 Uniprot

研究背景

功能:

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

亚基结构:

Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.

蛋白家族:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

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