Phospho-MeCP2 (Ser80) Antibody - #AF2435

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产品: 磷酸化 MeCP2 (Ser80) 抗体
货号: AF2435
描述: Rabbit polyclonal antibody to Phospho-MeCP2 (Ser80)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 75kDa; 52kD(Calculated).
蛋白号: P51608
RRID: AB_2845448

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
Phospho-MeCP2 (Ser80) Antibody detects endogenous levels of MeCP2 only when phosphorylated at Ser80.
RRID:
AB_2845448
引用格式: Affinity Biosciences Cat# AF2435, RRID:AB_2845448.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;

抗原和靶标

免疫原:
Uniprot:

P51608(MECP2_HUMAN) >>Visit HPA database.

基因/基因ID:
MECP2(4204)
表达:
P51608 MECP2_HUMAN:

Present in all adult somatic tissues tested.

序列:
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P51608 作为底物

Site PTM Type Enzyme
S13 Phosphorylation
S53 Phosphorylation
S70 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation Q9H2X6 (HIPK2)
S86 Phosphorylation
R115 Methylation
S116 Phosphorylation
K119 Methylation
Y120 Phosphorylation
K130 Ubiquitination
S149 Phosphorylation
T158 Phosphorylation
R162 Methylation
S164 Phosphorylation
S166 Phosphorylation
S178 Phosphorylation
T184 Phosphorylation
T203 Phosphorylation
S204 Phosphorylation
K210 Methylation
S216 Phosphorylation
K219 Acetylation
K223 Ubiquitination
T228 Phosphorylation
S229 Phosphorylation
K267 Methylation
S274 Phosphorylation
K285 Acetylation
T308 Phosphorylation
T311 Phosphorylation
S313 Phosphorylation
K321 Acetylation
T327 Phosphorylation
S332 Phosphorylation
K347 Methylation
S349 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
S395 Phosphorylation
S423 Phosphorylation
S426 Phosphorylation
K449 Acetylation
T479 Phosphorylation
S486 Phosphorylation

研究背景

功能:

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

翻译修饰:

Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

细胞定位:

Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Present in all adult somatic tissues tested.

亚基结构:

Interacts with FNBP3 (By similarity). Interacts with CDKL5. Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1. Interacts with TBL1X; recruits TBL1X to the heterochromatin foci.

文献引用

1). The role of MeCP2 and the BDNF/TrkB signaling pathway in the stress resilience of mice subjected to CSDS. Psychopharmacology (PubMed: 35711008) [IF=3.4]

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