Phospho-Calnexin (Ser564) Antibody - #AF2438
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2438, RRID:AB_2845451.
展开/折叠
Calnexin; CALX_HUMAN; CANX; CNX; FLJ26570; Histocompatibility complex class I antigen binding protein p88; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90;
抗原和靶标
- P27824 CALX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGKWLLCMLLVLGTAIVEAHDGHDDDVIDIEDDLDDVIEEVEDSKPDTTAPPSSPKVTYKAPVPTGEVYFADSFDRGTLSGWILSKAKKDDTDDEIAKYDGKWEVEEMKESKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTPELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGIYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIPDPEAVKPDDWDEDAPAKIPDEEATKPEGWLDDEPEYVPDPDAEKPEDWDEDMDGEWEAPQIANPRCESAPGCGVWQRPVIDNPNYKGKWKPPMIDNPSYQGIWKPRKIPNPDFFEDLEPFRMTPFSAIGLELWSMTSDIFFDNFIICADRRIVDDWANDGWGLKKAADGAAEPGVVGQMIEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQTSGMEYKKTDAPQPDVKEEEEEKEEEKDKGDEEEEGEEKLEEKQKSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P27824 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T59 | O-Glycosylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| T66 | O-Glycosylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| R77 | Methylation | Uniprot | |
| T79 | O-Glycosylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| R125 | Methylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K134 | Acetylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K137 | Acetylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| T211 | Phosphorylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| K217 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K227 | Acetylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| R282 | Methylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| Y393 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K459 | Acetylation | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| Y514 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| T562 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | P27361 (MAPK3) , P06493 (CDK1) | Uniprot |
研究背景
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.
Endoplasmic reticulum membrane>Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232).
Interacts with MAPK3/ERK1 (By similarity). Interacts with KCNH2. Associates with ribosomes (By similarity). Interacts with SGIP1; involved in negative regulation of endocytosis (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins. Interacts with SERPINA2P/SERPINA2 and with the S and Z variants of SERPINA1. Interacts with PPIB. Interacts with ZNRF4. Interacts with SMIM22.
(Microbial infection) Interacts with HBV large envelope protein, isoform L.
(Microbial infection) Interacts with HBV large envelope protein, isoform M; this association may be essential for isoform M proper secretion.
Belongs to the calreticulin family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
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