产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3447, RRID:AB_2834842.
展开/折叠
I kappa B beta; I-kappa-B-beta; IkappaBbeta; IKB beta; IkB-B; IkB-beta; IKBB; IKBB_HUMAN; IkBbeta; NF kappa BIB; NF-kappa-B inhibitor beta; NF-kappa-BIB; Nfkbib; Thyroid receptor interacting protein 9; Thyroid receptor-interacting protein 9; TR interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9;
抗原和靶标
- Q15653 IKBB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15653 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S19 | Phosphorylation | O14920 (IKBKB) , Q15418 (RPS6KA1) | Uniprot |
| S23 | Phosphorylation | Q15418 (RPS6KA1) , O14920 (IKBKB) | Uniprot |
| Y161 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S315 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
Cytoplasm. Nucleus.
Expressed in all tissues examined.
Interacts with THRB (via ligand-binding domain). Interacts with RELA and REL (By similarity). Interacts with COMMD1. Interacts with inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2.
Belongs to the NF-kappa-B inhibitor family.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
限制条款
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