产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3494, RRID:AB_2834846.
展开/折叠
1300015D22Rik; 5''-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase beta-1 subunit; AAKB1_HUMAN; AMP-activated protein kinase beta subunit; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; AU021155; E430008F22; HAMPKb; MGC17785; PRKAB1; Protein kinase AMP activated non catalytic subunit beta 1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1;
抗原和靶标
- Q9Y478 AAKB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y478 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S25 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| T80 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K89 | Ubiquitination | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S138 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K157 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S170 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S177 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S180 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y187 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| K268 | Ubiquitination | Uniprot |
研究背景
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.
The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
Belongs to the 5'-AMP-activated protein kinase beta subunit family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.