SH3BP5 Antibody - #DF12738
| 产品: | SH3BP5 抗体 |
| 货号: | DF12738 |
| 描述: | Rabbit polyclonal antibody to SH3BP5 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 47 kDa; 50kD(Calculated). |
| 蛋白号: | O60239 |
| RRID: | AB_2845699 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12738, RRID:AB_2845699.
展开/折叠
3BP5_HUMAN; OTTHUMP00000208289; OTTHUMP00000208291; OTTHUMP00000208294; SAB; SH3 binding protein; SH3 domain binding protein 5 (BTK associated); SH3 domain binding protein 5; SH3 domain binding protein that preferentially associates with BTK; SH3 domain-binding protein 5; SH3 domain-binding protein that preferentially associates with BTK; SH3 domainbinding protein 5; SH3BP 5; SH3BP-5; Sh3bp5;
抗原和靶标
Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
- O60239 3BP5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAALKRSRSEEPAEILPPARDEEEEEEEGMEQGLEEEEEVDPRIQGELEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQRLLEDDKRQFDSAWQEMLNHATQRVMEAEQTKTRSELVHKETAARYNAAMGRMRQLEKKLKRAINKSKPYFELKAKYYVQLEQLKKTVDDLQAKLTLAKGEYKMALKNLEMISDEIHERRRSSAMGPRGCGVGAEGSSTSVEDLPGSKPEPDAISVASEAFEDDSCSNFVSEDDSETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLDLPSPVSLSEFGMMFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKANNNRGLSSSSGSGGSSKSQSSTSPEGQALENRMKQLSLQCSKGRDGIIADIKMVQIG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60239 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Methylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K240 | Methylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | P53778 (MAPK12) , P45984 (MAPK9) | Uniprot |
| S375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| K440 | Ubiquitination | Uniprot |
研究背景
Functions as guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25. Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
Cytoplasmic vesicle membrane>Peripheral membrane protein. Mitochondrion.
Note: Colocalizes with RAB11A on cytoplasmic vesicle membranes.
Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
Interacts with BTK. Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12. Interacts with GDP-bound and nucleotide-free forms of RAB11A.
The N-terminal half of the protein mediates interaction with RAB11A and functions as guanine nucleotide exchange factor. Four long alpha-helices (interrupted by a central kink) assemble into coiled coils, giving rise to a 'V' shape.
Belongs to the SH3BP5 family.
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