产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3430, RRID:AB_2834872.
展开/折叠
DOD; DODO, Drosophila, homolog of; FLJ40239; FLJ77628; MGC10717; NIMA interacting 1; Peptidyl prolyl cis trans isomerase NIMA interacting 1; Peptidyl prolyl cis/trans isomerase NIMA interacting; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase pin1; Peptidylprolyl cis/trans isomerase NIMA interacting 1; Pin 1; Pin1; PIN1_HUMAN; PPIase Pin1; Prolyl isomerase; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1; Protein NIMA interacting 1; Rotamase Pin1; UBL 5; UBL5;
抗原和靶标
The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
- Q13526 PIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13526 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Sumoylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| S16 | Phosphorylation | P41279 (MAP3K8) , O14965 (AURKA) , P17612 (PRKACA) | Uniprot |
| Y23 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Sumoylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S65 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S71 | Phosphorylation | P53355 (DAPK1) | Uniprot |
| S72 | Phosphorylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| K82 | Sumoylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Q16584 (MAP3K11) | Uniprot |
| R142 | Methylation | Uniprot |
研究背景
Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes Ref. 21). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner. Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN. May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).
Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation.
Nucleus. Nucleus speckle. Cytoplasm.
Note: Colocalizes with NEK6 in the nucleus (PubMed:16476580). Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization (PubMed:21497122).
The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation. Directly interacts with RBBP8/CtIP; this interaction depends upon RBBP8 phosphorylation.
The WW domain is required for the interaction with STIL and KIF20B.
研究领域
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.