产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3451, RRID:AB_2834889.
展开/折叠
BSP-1; BSP1; HsMAD1; JV4-1; JV41; MAD homolog 1; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; Mad-related protein 1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMA- AND MAD-RELATED PROTEIN 1; SMAD 1; SMAD family member 1; SMAD mothers against DPP homolog 1; Smad1; SMAD1_HUMAN; TGF beta signaling protein 1; Transforming growth factor-beta-signaling protein 1;
抗原和靶标
- Q15797 SMAD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15797 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S11 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| K116 | Sumoylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K118 | Sumoylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | P50750 (CDK9) , P49336 (CDK8) , P28482 (MAPK1) | Uniprot |
| S195 | Phosphorylation | P49336 (CDK8) , P50750 (CDK9) , P28482 (MAPK1) | Uniprot |
| T202 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S206 | Phosphorylation | P50750 (CDK9) , P28482 (MAPK1) , P49336 (CDK8) , P50613 (CDK7) | Uniprot |
| S210 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | P28482 (MAPK1) , P50750 (CDK9) , P49336 (CDK8) | Uniprot |
| S239 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K269 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Q8N4C8 (MINK1) , Q9UKE5 (TNIK) | Uniprot |
| K418 | Ubiquitination | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | O00238 (BMPR1B) | Uniprot |
| S463 | Phosphorylation | Q05655 (PRKCD) , P36894 (BMPR1A) , O00238 (BMPR1B) | Uniprot |
| S465 | Phosphorylation | Q05655 (PRKCD) , O00238 (BMPR1B) , P36894 (BMPR1A) | Uniprot |
研究背景
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4.
Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity).
Cytoplasm. Nucleus.
Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity).
Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Found in a complex with SMAD4 and YY1. Interacts with HGS, NANOG and ZCCHC12 (By similarity). Upon C-terminus phosphorylation: forms trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2, USP15 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with SKOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation. Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Found in a macromolecular complex with FAM83G. Interacts (via MH2 domain) with FAM83G (via MH2 domain); in a SMAD4-independent manner. Interacts with ZC3H3 (By similarity). Interacts with TMEM119 (By similarity). Interacts (via MH1 and MH2 domains) with ZNF8 (By similarity). Interacts with RANBP3L; the interaction increases when SMAD1 is not phosphorylated and mediates SMAD1 nuclear export.
The MH2 domain mediates phosphorylation-dependent trimerization through L3 loop binding of phosphoserines in the adjacent subunit.
Belongs to the dwarfin/SMAD family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
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