产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3458, RRID:AB_2834896.
展开/折叠
CDC 37; Cdc37; CDC37 cell division cycle 37 homolog; CDC37 cell division cycle 37 S cerevisiae homolog; CDC37 cell division cycle 37, S cerevisiae, homolog of; Cdc37 homolog; CDC37 protein; CDC37_HUMAN; CDC37A; cell division cycle 37; Cell division cycle 37 homolog; Hsp90 chaperone protein kinase targeting subunit; Hsp90 chaperone protein kinase targeting subunit p50Cdc37; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co chaperone Cdc37; Hsp90 co-chaperone Cdc37; p50; p50Cdc37; S cerevisiae hypothetical protein CDC37;
抗原和靶标
- Q16543 CDC37_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVAHQTIVMQFILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMKEYEEEERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVQMLQDAISKMDPTDAKYHMQRCIDSGLWVPNSKASEAKEGEEAGPGDPLLEAVPKTGDEKDVSV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16543 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| V2 | Acetylation | Uniprot | |
| Y4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) | Uniprot |
| K45 | Acetylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| K132 | Sumoylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| R246 | Methylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | P07947 (YES1) | Uniprot |
| S300 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| C308 | S-Nitrosylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K330 | Acetylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| C336 | S-Nitrosylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| T370 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot |
研究背景
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Inhibits HSP90AA1 ATPase activity.
Constitutively sumoylated by UBE2I.
Cytoplasm.
Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex with Hsp90/HSP90AB1 and CDK6. Interacts with HSP90AA1. Interacts with AR, CDK4, CDK6 and EIF2AK1. Interacts with RB1 (By similarity). Interacts with KSR1. Interacts with FLCN, FNIP1 and FNIP2.
Belongs to the CDC37 family.
研究领域
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
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