Phospho-MLC2 (Ser15) Antibody - #AF8618

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产品: 磷酸化 MLC2 (Ser15) 抗体
货号: AF8618
描述: Rabbit polyclonal antibody to Phospho-MLC2 (Ser15)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Sheep, Rabbit, Dog, Chicken
分子量: 18kDa; 19kD(Calculated).
蛋白号: P10916
RRID: AB_2846214

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:200-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-MLC2 (Ser15) Antibody detects endogenous levels of MLC2 only when phosphorylated at Ser15.
RRID:
AB_2846214
引用格式: Affinity Biosciences Cat# AF8618, RRID:AB_2846214.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cardiac myosin light chain-2;Cardiac ventricular myosin light chain 2;CMH10;MLC 2v;MLC-2;MLC-2v;MLC2;MLRV_HUMAN;MYL 2;MYL2;Myosin light chain 2 regulatory cardiac slow;Myosin light polypeptide 2 regulatory cardiac slow;Myosin regulatory light chain 2;Myosin regulatory light chain 2 ventricular/cardiac muscle isoform;Regulatory light chain of myosin;RLC of myosin;Slow cardiac myosin regulatory light chain 2;ventricular/cardiac muscle isoform;

抗原和靶标

免疫原:
Uniprot:

P10916(MLRV_HUMAN) >>Visit HPA database.

基因/基因ID:
MYL2(4633)
序列:
MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
75
Pig
0
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10916 作为底物

Site PTM Type Enzyme
S15 Phosphorylation Q15746 (MYLK)
S19 Phosphorylation Q5VT25 (CDC42BPA)
T80 Phosphorylation
T98 Phosphorylation
K104 Acetylation
K111 Acetylation
Y118 Phosphorylation
T124 Phosphorylation
Y152 Phosphorylation
K153 Acetylation
K165 Acetylation

研究背景

功能:

Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

翻译修饰:

N-terminus is methylated by METTL11A/NTM1.

Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).

细胞定位:

Cytoplasm>Myofibril>Sarcomere>A band.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes. Bioactive Materials, 2022 (PubMed: 35387166) [IF=18.9]

Application: WB    Species: rat    Sample: RAW 264.7 cells

Fig. S16. | Verification of transcriptome analysis of RAW 264.7 cells cultured on various scaffolds. The mRNA expression levels of (a) glycolytic and (b) RhoA/ROCK pathways-related genes in each group. (c) Western blot (WB) analysis of glycolytic and RhoA/ROCK pathways-related proteins HIF-1α, Pdk1, Pgk1, Cdc42, Rac1, and p-MLC/MLC in each group.
2). Protective effect of oxyberberine against acute lung injury in mice via inhibiting RhoA/ROCK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35753262) [IF=7.5]
3). Walnut-Derived Peptide Improves Cognitive Impairment in Colitis Mice Induced by Dextran Sodium Sulfate via the Microbiota-Gut-Brain Axis (MGBA). Journal of agricultural and food chemistry, 2023 (PubMed: 38039336) [IF=6.1]
4). β-patchoulene simultaneously ameliorated dextran sulfate sodium-induced colitis and secondary liver injury in mice via suppressing colonic leakage and flora imbalance. Biochemical Pharmacology, 2020 (PubMed: 33017576) [IF=5.8]

Application: WB    Species: mouse    Sample: colon

Fig.4.| Effect of β-PAE on TLR4/MyD88/NF-κB and ROCK/MLC signalling pathway.(A) Representative bands of TLR4, MyD88, phospho-IκBα, IκBα and p65. (B)Quantitative results of band densities of TLR4, MyD88, phospho-IκBα, IκBα and p65.(C) Representative bands of ROCK1, phospho-MLC2 and MLC2.

Application: WB    Species: mouse    Sample: Colons

Fig.4. |Effect of β-PAE on TLR4/MyD88/NF-κB and ROCK/MLC signalling pathway. (C) Representative bands of ROCK1, phospho-MLC2 and MLC2. (D) Quantitative results of band densities of ROCK1, phospho-MLC2 and MLC2.
5). Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis. Bioengineered, 2022 (PubMed: 35324411) [IF=4.9]

Application: WB    Species: Mouse    Sample: RAW264.7 and Ana-1 cells

Figure 2. Protein levels of RhoA, ROCK1, ROCK2 and p-MLC in RAW264.7 and Ana-1 cells. *P < 0.05 vs. Control group, #P < 0.05 vs. AEm group.
6). An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via the ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota. Pharmaceuticals, 2024 [IF=4.6]

Application: WB    Species: Rat    Sample: IEC-6 cells

Figure 4. Effects of CP-A on ERK/MLCK/MLC2 signaling pathway proteins in 5-FU-induced rats and IEC-6 cells: (a–d) Expression of MLC2, p-MLC2, MLCK, ERK1/2 and p-ERK1/2 in IEC-6 cells. (e) MLCK protein in IEC-6 cells (magnification 100×). (f–i) Expression of MLC2, p-MLC2, MLCK, ERK1/2 and p-ERK1/2 in intestinal. (j) The p-MLC2 protein in IEC-6 cells (magnification 100×). Values are represented as mean ± SEM (n = 3); ## p < 0.01 versus control group, * p < 0.05 and ** p < 0.01 versus 5-FU group.

Application: IHC    Species: Rat    Sample: IEC-6 cells

Figure 4. Effects of CP-A on ERK/MLCK/MLC2 signaling pathway proteins in 5-FU-induced rats and IEC-6 cells: (a–d) Expression of MLC2, p-MLC2, MLCK, ERK1/2 and p-ERK1/2 in IEC-6 cells. (e) MLCK protein in IEC-6 cells (magnification 100×). (f–i) Expression of MLC2, p-MLC2, MLCK, ERK1/2 and p-ERK1/2 in intestinal. (j) The p-MLC2 protein in IEC-6 cells (magnification 100×). Values are represented as mean ± SEM (n = 3); ## p < 0.01 versus control group, * p < 0.05 and ** p < 0.01 versus 5-FU group.
7). Disruption of the lung-gut-brain axis is responsible for cortex damage induced by pulmonary exposure to zinc oxide nanoparticles. TOXICOLOGY, 2023 (PubMed: 36535435) [IF=4.5]
8). Sarcomere length affects Ca2+ sensitivity of contraction in ischemic but not non-ischemic myocardium. JOURNAL OF GENERAL PHYSIOLOGY, 2023 (PubMed: 36633584) [IF=3.8]
9). Inhibition of Peripheral ERK Signaling Ameliorates Persistent Muscle Pain Around Trigger Points in Rats. Cell Transplantation, 2020 (PubMed: 33081508) [IF=3.3]

Application: WB    Species: rat    Sample: muscle

Figure 6. |MLCK might be the target of the ERK inhibitor involved in ameliorating muscular mechanical hyperalgesia. (C) The expression of p-MLC protein. p-MLC expression was increased in the MPS group compared with that in the control group. p-MLC expression was decreased in the ERK inhibitor group compared with that in the MPS group.
10). RHOA inhibits chondrogenic differentiation of mesenchymal stem cells in adolescent idiopathic scoliosis. CONNECTIVE TISSUE RESEARCH, 2022 (PubMed: 35019797) [IF=2.9]
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