(6)
(10)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13303, RRID:AB_2846322.
展开/折叠
CMD1LL; KIAA1675; LVNC8; MDS1/EVI1 like gene 1; MDS1/EVI1-like gene 1; MEL1; PFM 13; PFM13; PR domain containing 16; PR domain containing protein 16; PR domain zinc finger protein 16; PR domain-containing protein 16; PRD16_HUMAN; Prdm16; Transcription factor MEL 1; Transcription factor MEL1;
抗原和靶标
Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells.
- Q9HAZ2 PRD16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRSKARARKLAKSDGDVVNNMYEPNRDLLASHSAEDEAEDSAMSPIPVGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPADFELRESSIPGAGLGVWAKRKMEAGERLGPCVVVPRAAAKETDFGWEQILTDVEVSPQEGCITKISEDLGSEKFCVDANQAGAGSWLKYIRVACSCDDQNLTMCQISEQIYYKVIKDIEPGEELLVHVKEGVYPLGTVPPGLDEEPTFRCDECDELFQSKLDLRRHKKYTCGSVGAALYEGLAEELKPEGLGGGSGQAHECKDCERMFPNKYSLEQHMVIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGGIFAPGLPLTPSPMMDKAKPSPSLNHASLGFNEYFPSRPHPGSLPFSTAPPTFPALTPGFPGIFPPSLYPRPPLLPPTSLLKSPLNHTQDAKLPSPLGNPALPLVSAVSNSSQGTTAAAGPEEKFESRLEDSCVEKLKTRSSDMSDGSDFEDVNTTTGTDLDTTTGTGSDLDSDVDSDPDKDKGKGKSAEGQPKFGGGLAPPGAPNSVAEVPVFYSQHSFFPPPDEQLLTATGAAGDSIKAIASIAEKYFGPGFMGMQEKKLGSLPYHSAFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKDVKPILPMPKGPSAPASGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKLGAGEGLPQVCPARMPQQPPLHYAKPSPFFMDPIYSRVEKRKVTDPVGALKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHENAPVSQHPGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRTEKRADMQIVDGSAQCPGLASEKQEDVEEEDDDDLEEDDEDSLAGKSQDDTVSPAPEPQAAYEDEEDEEPAASLAVGFDHTRRCAEDHEGGLLALEPMPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEALKHTLCRQAKNQAYAMMLSLSEDTPLHTPSQGSLDAWLKVTGATSESGAFHPINHL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9HAZ2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S44 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| Y193 | Phosphorylation | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| K321 | Sumoylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| K382 | Sumoylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| T876 | Phosphorylation | Uniprot | |
| K915 | Acetylation | Uniprot | |
| T974 | Phosphorylation | Uniprot | |
| S989 | Phosphorylation | Uniprot | |
| S1089 | Phosphorylation | Uniprot | |
| T1090 | Phosphorylation | Uniprot | |
| K1194 | Acetylation | Uniprot |
研究背景
Binds DNA and functions as a transcriptional regulator. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (By similarity). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (By similarity). Functions as a repressor of TGF-beta signaling.
Binds DNA and functions as a transcriptional regulator. Functions as a repressor of TGF-beta signaling. May regulate granulocyte differentiation.
Nucleus. Cytoplasm.
Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells.
Interacts with CEBPA, CEBPB and CEBPD; the interaction is direct. Interacts with PPARG and PPARA; controls brown adipocytes differentiation. Interacts with CTBP1 and CTBP2; represses the expression of WAT-specific genes. Interacts with PPARGC1A and PPARGC1B; interaction with PPARGC1A or PPARGC1B activates the transcription of BAT-specific gene (By similarity). Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. Interacts with ZNF516; the interaction is direct and may play a role in the transcription of brown adipose tissue-specific gene.
Belongs to the PRDM16 family.
文献引用
Application: WB Species: mouse Sample: white adipose
Application: WB Species: mouse Sample: white adipose
Application: WB Species: Mice Sample: adipose tissue
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.