CD20 Antibody - #DF13319

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产品: CD20 抗体
货号: DF13319
描述: Rabbit polyclonal antibody to CD20
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Bovine, Sheep, Dog
分子量: 33kDa; 33kD(Calculated).
蛋白号: P11836
RRID: AB_2846338

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
克隆:
Polyclonal
特异性:
CD20 Antibody detects endogenous levels of total CD20.
RRID:
AB_2846338
引用格式: Affinity Biosciences Cat# DF13319, RRID:AB_2846338.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APY; ATOPY; B lymphocyte antigen CD20; B Lymphocyte Cell Surface Antigen B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; B1; Bp 35; Bp35; CD 20; CD20; CD20 antigen; CD20 receptor; CD20_HUMAN; CVID5; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU 16; LEU16; leukocyte surface antigen Leu 16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains A1; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1; MGC3969; MS4A1; MS4A2; S7;

抗原和靶标

免疫原:
Uniprot:

P11836(CD20_HUMAN) >>Visit HPA database.

基因/基因ID:
MS4A1(931)
表达:
P11836 CD20_HUMAN:

Expressed on B-cells.

序列:
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
88
Bovine
88
Sheep
88
Dog
88
Rabbit
75
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11836 作为底物

Site PTM Type Enzyme
T2 Phosphorylation
T3 Phosphorylation
S7 Phosphorylation
T11 Phosphorylation
S25 Phosphorylation
S35 Phosphorylation
S36 Phosphorylation
T41 Phosphorylation
S121 Phosphorylation
S225 Phosphorylation
S231 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
K235 Ubiquitination
K236 Ubiquitination
K243 Ubiquitination
T250 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S253 Phosphorylation
S254 Phosphorylation
S289 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

研究背景

功能:

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

翻译修饰:

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell membrane>Lipid-anchor.
Note: Constitutively associated with membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed on B-cells.

亚基结构:

Forms homotetramers. Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR.

蛋白家族:

Belongs to the MS4A family.

研究领域

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

文献引用

1). Dupilumab improve acquired reactive perforating collagenosis characterized by type 2 inflammation. Frontiers in Immunology (PubMed: 37638036) [IF=7.3]

Application: IHC    Species: Human    Sample:

Figure 3 Representative images and data reporting hematoxylin and eosin staining, CD3, CD20, GATA3 and T-bet staining of APRC, AD and HC samples. Magnification: ×40, ×200, AD, atopic dermatitis, ARPC, acquired reactive perforating collagenosis, HC, healthy control, HE, hematoxylin and eosin.
2). Pyruvate dehydrogenase kinase 4‐mediated metabolic reprogramming is involved in rituximab resistance in diffuse large B‐cell lymphoma by affecting the expression of MS4A1/CD20. CANCER SCIENCE (PubMed: 34252986) [IF=5.7]

Application: IF/ICC    Species: Human    Sample: DLBCL cells

FIGURE 4 Pyruvate dehydrogenase kinase 4 (PDK4) has a negative regulatory effect on MS4A1/CD20 expression in diffuse large B- cell lymphoma cells. A, B, Real- time quantitative PCR analysis of PDK4 and MS4A1 mRNA expression in U2932 and OCI- Ly8 cells transfected with PDK4 overexpressing (PDK4 OE) plasmid or empty vector (EV). C, D, CD20 (green) molecules in the plasma membrane showed a reduction after PDK4 overexpression in both U2932 and OCI- Ly8 cells. Scale bar, 15 μm. E, F, Western blot analysis of PDK4 and CD20 protein levels in U2932 and OCI- Ly8 cells with PDK4 shRNA interference or transfected with PDK4 OE plasmid or EV. *P < .05, **P < .01, ***P < .001

Application: WB    Species: Human    Sample: DLBCL cells

FIGURE 2 High pyruvate dehydrogenase kinase 4 (PDK4) is associated with rituximab (RTX) resistance and low MS4A1/CD20 in diffuse large B- cell lymphoma (DLBCL) cells. A, B, Real- time quantitative PCR analysis of PDK4 and MS4A1 mRNA expression in DLBCL cell lines U2932, OCI- ly7, and OCI- ly8. C, Western blot analysis of PDK4 and CD20 protein levels in DLBCL cell lines U2932, OCI- ly7, and OCI- ly8. D, E, Annexin V- phycoerythrin (PE)/7- AAD double staining analysis of the three DLBCL cell lines treated with RTX (50 μg/mL). F, G, Mitochondrial membrane potential of DLBCL cells following treatment with RTX for 48 hours and stained with JC- 1 probe. Representative pictures of JC- 1 staining are shown. Scale bar, 25 μm. ***P < .001
3). PDK4-Mediated Metabolic Reprogramming Promotes Rituximab Resistance in Diffuse Large B-Cell Lymphoma Via Negative Regulation of MS4A1/CD20. Research Square

Application: WB    Species: Human    Sample: DLBCL cells

Figure 2. High PDK4 is associated with rituximab (RTX) resistance and low MS4A1/CD20 in DLBCL cells. A and B, qRT-PCR analysis of PDK4 and MS4A1 mRNA expression in DLBCL cell lines U2932, OCI-ly7 and OCI-ly8. C, western blotting analysis of PDK4 and CD20 protein levels in DLBCL cell lines U2932, OCI-ly7 and OCI-ly8. D and E, annexin V-FITC/PI double staining analysis of the three DLBCL cell lines treated with rituximab (50 μg/ml).

Application: IF/ICC    Species: Human    Sample: DLBCL cells

Figure 2. High PDK4 is associated with rituximab (RTX) resistance and low MS4A1/CD20 in DLBCL cells. A and B, qRT-PCR analysis of PDK4 and MS4A1 mRNA expression in DLBCL cell lines U2932, OCI-ly7 and OCI-ly8. C, western blotting analysis of PDK4 and CD20 protein levels in DLBCL cell lines U2932, OCI-ly7 and OCI-ly8. D and E, annexin V-FITC/PI double staining analysis of the three DLBCL cell lines treated with rituximab (50 μg/ml).

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