Phospho-HDAC5 (Ser498) Antibody - #AF3491

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产品: 磷酸化 HDAC5 (Ser498) 抗体
货号: AF3491
描述: Rabbit polyclonal antibody to Phospho-HDAC5 (Ser498)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 124kDa; 122kD(Calculated).
蛋白号: Q9UQL6
RRID: AB_2834929

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-HDAC5 (Ser498) Antibody detects endogenous levels of HDAC5 only when phosphorylated at Serine 498.
RRID:
AB_2834929
引用格式: Affinity Biosciences Cat# AF3491, RRID:AB_2834929.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen NY CO 9; Antigen NY-CO-9; HD5; HDAC 5; HDAC5; HDAC5_HUMAN; Histone deacetylase 5; NY CO 9;

抗原和靶标

免疫原:
Uniprot:

Q9UQL6(HDAC5_HUMAN) >>Visit HPA database.

基因/基因ID:
HDAC5(10014)
表达:
Q9UQL6 HDAC5_HUMAN:

Ubiquitous.

描述:
HDAC9 a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4.
序列:
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
91
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UQL6 作为底物

Site PTM Type Enzyme
S3 Phosphorylation
S7 Phosphorylation
K35 Sumoylation
S53 Phosphorylation
S55 Phosphorylation
S66 Phosphorylation
T70 Phosphorylation
K84 Ubiquitination
K115 Ubiquitination
K136 Ubiquitination
S180 Phosphorylation
T181 Phosphorylation
S206 Phosphorylation
S220 Phosphorylation
T234 Phosphorylation
K256 Ubiquitination
S259 Phosphorylation O43318 (MAP3K7) , Q05655 (PRKCD) , Q13555 (CAMK2G) , Q7KZI7 (MARK2) , Q9UQM7 (CAMK2A) , Q16566 (CAMK4) , P57059 (SIK1) , Q15139 (PRKD1) , Q13131 (PRKAA1) , Q14012 (CAMK1) , P54646 (PRKAA2)
S278 Phosphorylation Q96GD4 (AURKB)
S279 Phosphorylation Q9Y463 (DYRK1B) , P17612 (PRKACA) , Q00535 (CDK5)
T288 Phosphorylation
S291 Phosphorylation
T292 Phosphorylation Q16512 (PKN1)
S368 Phosphorylation
S469 Phosphorylation
T496 Phosphorylation
S498 Phosphorylation P54646 (PRKAA2) , Q14012 (CAMK1) , Q16566 (CAMK4) , Q13555 (CAMK2G) , Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , P57059 (SIK1) , Q15139 (PRKD1) , O94806 (PRKD3) , Q13131 (PRKAA1) , Q9BZL6 (PRKD2)
S504 Phosphorylation
K533 Acetylation
K533 Sumoylation
K537 Ubiquitination
T546 Phosphorylation
T547 Phosphorylation
K605 Sumoylation
S611 Phosphorylation
S661 Phosphorylation
S671 Phosphorylation
S755 Phosphorylation
Y776 Phosphorylation
S798 Phosphorylation
S799 Phosphorylation
S1071 Phosphorylation
S1108 Phosphorylation

研究背景

功能:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.

翻译修饰:

Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).

Ubiquitinated. Polyubiquitination however does not lead to its degradation.

细胞定位:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Interacts with AHRR, BAHD1, BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with BCL6, DDIT3/CHOP, GRK5, KDM5B and MYOCD. Interacts with EP300 in the presence of TFAP2C. Interacts with ANKRA2. Interacts with CUL7 (as part of the 3M complex); negatively regulated by ANKRA2. Interacts with ZBTB7B; the interaction allows the recruitment of HDAC4 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression (By similarity). Interacts with RARA.

蛋白家族:

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Belongs to the histone deacetylase family. HD type 2 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Wilson disease: intersecting DNA methylation and histone acetylation regulation of gene expression in a mouse model of hepatic copper accumulation. Cellular and Molecular Gastroenterology and Hepatology, 2021 (PubMed: 34098115) [IF=7.2]

Application: WB    Species: Mice    Sample: tx-j

Figure 2. Expression of HDAC4 and HDAC5 in mouse and HepG2 models of WD. Immunoblot densitometry analyses are normalized to β-ACTIN; data are represented as means ± SEM and statistical significance was determined by Student's t-test (*p< 0.05, **p< 0.01, and ****p< 0.0001). A: Total protein liver lysate densitometries for HDAC4 and HDAC5 protein expression in tx-j mice compared to C3H control at post-partum day 6 (PPD6; C3H n=4M/4F, tx-j n=5M/4F), 9 weeks (C3H n=4M/3F, tx-j n=4M/4F), 12 weeks (C3H n=4M/4F, tx-j n=4M/3F), and 24 weeks (C3H n=10M/12F, tx-j n=11M/11F). B: Total protein liver lysate densitometry analyses for HDAC4 and HDAC5 protein expression in 16-week old Atp7b -/- mice (n=7M/4F) and wild-type (WT, n=6M/5F). C: Immunohistochemical analysis of 24-week old C3H and tx-j mouse livers for HDAC5 (red) and DAPI (blue). Images display cytosolic and nuclear HDAC5 localization. White arrows indicate nuclei; scale bar = 50 µm. Bar graphs represent HDAC5 optical density in cytosol, nuclei, and the nucleus/cytosol ratio; n=3 mice/group, 60 cells/mouse. D: HDAC5 immunoblot of HepG2 cell lysates treated with CuSO 4 (0 to 100 µM; n=3 per treatment) for 24 hours. In this figure and all subsequent figures: C3H=C3HeB/FeJ control mice with normal copper metabolism, tx-j=C3He- Atp7b tx-j /J Jackson Laboratory toxic milk mouse model of Wilson disease (WD).
2). Antidepressant effect and mechanism of TMP269 on stress-induced depressive-like behavior in mice. Biochemical pharmacology, 2024 (PubMed: 38801927) [IF=5.8]
3). WNT10A induces apoptosis of senescent synovial resident stem cells through Wnt/calcium pathway-mediated HDAC5 phosphorylation in OA joints. BONE, 2021 (PubMed: 34000432) [IF=4.1]

Application: WB    Species: human    Sample: Sn-SMSCs

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phosphorylation at s498 (right); *P<0.05

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