Phospho-DNMT3A (Ser390/Ser393) Antibody - #AF3503
| 产品: | 磷酸化 DNMT3A (Ser390/Ser393) 抗体 |
| 货号: | AF3503 |
| 描述: | Rabbit polyclonal antibody to Phospho-DNMT3A (Ser390/Ser393) |
| 应用: | IHC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 100~130kD; 102kD(Calculated). |
| 蛋白号: | Q9Y6K1 |
| RRID: | AB_2846817 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3503, RRID:AB_2846817.
展开/折叠
DNA (cytosine 5) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3 alpha; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNM3A_HUMAN; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M.HsaIIIA; MCMT; OTTHUMP00000201149; TBRS;
抗原和靶标
A synthesized peptide derived from human DNMT3A around the phosphorylation site of Ser390/Ser393.
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
- Q9Y6K1 DNM3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAGMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAVEVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV
翻译修饰 - Q9Y6K1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R23 | Methylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| R171 | Methylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K468 | Sumoylation | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| T645 | Phosphorylation | Uniprot | |
| K721 | Ubiquitination | Uniprot | |
| K783 | Ubiquitination | Uniprot | |
| K812 | Ubiquitination | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| K844 | Ubiquitination | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| K906 | Ubiquitination | Uniprot |
研究背景
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Nucleus. Cytoplasm.
Note: Accumulates in the major satellite repeats at pericentric heterochromatin.
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2 (By similarity). Binds the ZBTB18 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4).
The PWWP domain is essential for targeting to pericentric heterochromatin. It specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3) (By similarity).
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
研究领域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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