产品: 磷酸化 STUB1/CHIP (Ser19) 抗体
货号: AF3514
描述: Rabbit polyclonal antibody to Phospho-STUB1/CHIP (Ser19)
应用: IHC
反应: Human
分子量: 35kD(Calculated).
蛋白号: Q9UNE7
RRID: AB_2846828

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
Phospho-CHIP (Ser19) Antibody detects endogenous levels of CHIP only when phosphorylated at Ser19.
RRID:
AB_2846828
引用格式: Affinity Biosciences Cat# AF3514, RRID:AB_2846828.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen NY CO 7; Antigen NY-CO-7; C terminus of Hsp70-interacting protein; Carboxy terminus of Hsp70 interacting protein; Carboxy terminus of Hsp70-interacting protein; Carboxy terminus of Hsp70p interacting protein; CHIP; CHIP_HUMAN; CLL associated antigen KW 8; CLL-associated antigen KW-8; E3 ubiquitin protein ligase CHIP; E3 ubiquitin-protein ligase CHIP; Heat shock protein A binding protein 2 (c terminal); HSPABP2; NY CO 7; PP1131; SDCCAG7; Serologically defined colon cancer antigen 7; STIP1 homology and U Box containing protein 1; STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase; STIP1 homology and U box-containing protein 1; STUB 1; STUB1; UBOX 1; UBOX1;

抗原和靶标

免疫原:

A synthesized peptide derived from human CHIP around the phosphorylation site of Ser19.

Uniprot:
基因/基因ID:
表达:
Q9UNE7 CHIP_HUMAN:

Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta (PubMed:10330192, PubMed:11435423). Detected in kidney, liver and lung (PubMed:10330192, PubMed:11435423).

序列:
MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY

翻译修饰 - Q9UNE7 作为底物

Site PTM Type Enzyme
K2 Methylation
K2 Ubiquitination
S19 Phosphorylation
K22 Ubiquitination
S23 Phosphorylation
S25 Phosphorylation
K30 Sumoylation
K30 Ubiquitination
R35 Methylation
K41 Methylation
K41 Sumoylation
K41 Ubiquitination
C48 S-Nitrosylation
Y49 Phosphorylation
K72 Methylation
K125 Sumoylation
K125 Ubiquitination
S137 Phosphorylation
S149 Phosphorylation
C180 S-Nitrosylation
C199 S-Nitrosylation
K203 Sumoylation
K203 Ubiquitination
Y207 Phosphorylation
K221 Ubiquitination
K255 Sumoylation
K255 Ubiquitination
T271 Phosphorylation
S273 Phosphorylation
T276 Phosphorylation

研究背景

功能:

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin-mediated protein degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. Likely mediates polyubiquitination and downregulates plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation. May regulate myosin assembly in striated muscles together with UBE4B and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation. Mediates ubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation.

翻译修饰:

Monoubiquitinated at Lys-2 following cell stress by UBE2W, promoting the interaction with ATXN3 (By similarity). Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.

细胞定位:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus in response to inflammatory signals in regulatory T-cells (Treg).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in differentiated myotubes (at protein level). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.

亚基结构:

Homodimer (By similarity). Interacts with BAG2. Interacts with E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with MKKS. Interacts with DNAAF4. Interacts with POLB. Interacts (when monoubiquitinated) with ATXN3. Interacts with UBE2W. Interacts (via the U-box domain) with the UBE2V2-UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity (By similarity). Interacts with DNAJB6. Interacts with FOXP3. Interacts with FLCN. Interacts with HSP90AA1. Interacts with HSP90. Interacts with UBE2N and UBE2V1. Interacts (via TPR repeats) with the C-terminal domain of HSPA1A. Interacts with the non-acetylated form of HSPA1A and HSPA1B. Interacts (via TPR repeats) with the C-terminal domain of HSPA8. Interacts with SMAD3 and HSP90AB1. Interacts with UBE4B. Interacts with RIPK3.

蛋白家族:

The U-box domain is required for the ubiquitin protein ligase activity.

The TPR domain is essential for ubiquitination mediated by UBE2D1.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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