产品: 磷酸化 TAO2 (Ser181) 抗体
货号: AF3516
描述: Rabbit polyclonal antibody to Phospho-TAO2 (Ser181)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 138kD(Calculated).
蛋白号: Q9UL54
RRID: AB_2846830

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-TAO2 (Ser181) Antibody detects endogenous levels of TAO2 only when phosphorylated at Ser181.
RRID:
AB_2846830
引用格式: Affinity Biosciences Cat# AF3516, RRID:AB_2846830.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1110033K02Rik; B230344N16; hKFC C; hKFC-C; KIAA0881; Kinase from chicken homolog C; MAP3K17; mKIAA0881; Prostate derived STE20 like kinase 1; Prostate derived STE20 like kinase PSK; Prostate derived sterile 20 like kinase 1; Prostate-derived STE20-like kinase 1; PSK 1; PSK; PSK-1; PSK1; PSK1 beta; Serine/threonine protein kinase TAO2; Serine/threonine-protein kinase TAO2; TAO 1; TAO 2; TAO kinase 2; TAO1; TAO2; TAOK 2; Taok2; TAOK2_HUMAN; Thousand and one amino acid protein 2; Thousand and one amino acid protein kinase; UNQ2971/PRO7431;

抗原和靶标

免疫原:

A synthesized peptide derived from human TAO2 around the phosphorylation site of Ser181.

Uniprot:
基因/基因ID:
表达:
Q9UL54 TAOK2_HUMAN:

Ubiquitously expressed, with a higher level of expression in testis and brain.

序列:
MPAGGRAGSLKDPDVAELFFKDDPEKLFSDLREIGHGSFGAVYFARDVRNSEVVAIKKMSYSGKQSNEKWQDIIKEVRFLQKLRHPNTIQYRGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAVTHGALQGLAYLHSHNMIHRDVKAGNILLSEPGLVKLGDFGSASIMAPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWSLGITCIELAERKPPLFNMNAMSALYHIAQNESPVLQSGHWSEYFRNFVDSCLQKIPQDRPTSEVLLKHRFVLRERPPTVIMDLIQRTKDAVRELDNLQYRKMKKILFQEAPNGPGAEAPEEEEEAEPYMHRAGTLTSLESSHSVPSMSISASSQSSSVNSLADASDNEEEEEEEEEEEEEEEGPEAREMAMMQEGEHTVTSHSSIIHRLPGSDNLYDDPYQPEITPSPLQPPAAPAPTSTTSSARRRAYCRNRDHFATIRTASLVSRQIQEHEQDSALREQLSGYKRMRRQHQKQLLALESRLRGEREEHSARLQRELEAQRAGFGAEAEKLARRHQAIGEKEARAAQAEERKFQQHILGQQKKELAALLEAQKRTYKLRKEQLKEELQENPSTPKREKAEWLLRQKEQLQQCQAEEEAGLLRRQRQYFELQCRQYKRKMLLARHSLDQDLLREDLNKKQTQKDLECALLLRQHEATRELELRQLQAVQRTRAELTRLQHQTELGNQLEYNKRREQELRQKHAAQVRQQPKSLKVRAGQRPPGLPLPIPGALGPPNTGTPIEQQPCSPGQEAVLDQRMLGEEEEAVGERRILGKEGATLEPKQQRILGEESGAPSPSPQKHGSLVDEEVWGLPEEIEELRVPSLVPQERSIVGQEEAGTWSLWGKEDESLLDEEFELGWVQGPALTPVPEEEEEEEEGAPIGTPRDPGDGCPSPDIPPEPPPTHLRPCPASQLPGLLSHGLLAGLSFAVGSSSGLLPLLLLLLLPLLAAQGGGGLQAALLALEVGLVGLGASYLLLCTALHLPSSLFLLLAQGTALGAVLGLSWRRGLMGVPLGLGAAWLLAWPGLALPLVAMAAGGRWVRQQGPRVRRGISRLWLRVLLRLSPMAFRALQGCGAVGDRGLFALYPKTNKDGFRSRLPVPGPRRRNPRTTQHPLALLARVWVLCKGWNWRLARASQGLASHLPPWAIHTLASWGLLRGERPTRIPRLLPRSQRQLGPPASRQPLPGTLAGRRSRTRQSRALPPWR

翻译修饰 - Q9UL54 作为底物

Site PTM Type Enzyme
S9 Phosphorylation
K11 Ubiquitination
K21 Ubiquitination
K26 Ubiquitination
S29 Phosphorylation
S38 Phosphorylation
Y43 Phosphorylation
Y61 Phosphorylation
K64 Acetylation
K69 Acetylation
K69 Ubiquitination
Y107 Phosphorylation
K153 Ubiquitination
S181 Phosphorylation Q9UL54 (TAOK2)
K264 Ubiquitination
K277 Ubiquitination
Y309 Phosphorylation
K314 Ubiquitination
Y338 Phosphorylation
T344 Phosphorylation
S347 Phosphorylation
S414 Phosphorylation
Y426 Phosphorylation
Y430 Phosphorylation
S437 Phosphorylation
T450 Phosphorylation
S452 Phosphorylation
T468 Phosphorylation
S473 Phosphorylation
S486 Phosphorylation
K541 Ubiquitination
K552 Ubiquitination
K563 Ubiquitination
K573 Ubiquitination
S603 Phosphorylation
T604 Phosphorylation
K609 Ubiquitination
S656 Phosphorylation
K673 Ubiquitination
K722 Ubiquitination
K731 Ubiquitination
S777 Phosphorylation
S825 Phosphorylation
S827 Phosphorylation
R1126 Methylation
R1133 Methylation

翻译修饰 - Q9UL54 作为激酶

Substrate Site Source
P19419-1 (ELK1) S383 Uniprot
P46734 (MAP2K3) S218 Uniprot
P52564 (MAP2K6) S207 Uniprot
P52564 (MAP2K6) T211 Uniprot
Q9UL54 (TAOK2) S181 Uniprot

研究背景

功能:

Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation.

翻译修饰:

Isoforms 1 and 2 are autophosphorylated.

C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.

Autophosphorylated. Phosphorylated by ATM.

Phosphorylated on Ser-1031 by MAPK14. This phosphorylation is required PCDH8 for endocytosis (By similarity).

细胞定位:

Cytoplasmic vesicle membrane>Multi-pass membrane protein. Cytoplasm>Cytoskeleton. Nucleus.
Note: Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus.

Cell projection>Dendrite.
Note: In dendrites, colocalizes with PCDH8.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed, with a higher level of expression in testis and brain.

亚基结构:

Interacts with MAP2K3 and MAP2K6 (By similarity). Self-associates. Interacts with tubulins through the C-terminal domain. Interacts with MAP3K7 and interfers with MAP3K7-binding to CHUK and thus prevents NF-kappa-B activation. Isoform 2 interacts with PCDH8; this complex may also include CDH2 (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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