Phospho-PAK5/6 (Ser602/Ser560) Antibody - #AF3522

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产品: 磷酸化 PAK5/6 (Ser602/Ser560) 抗体
货号: AF3522
描述: Rabbit polyclonal antibody to Phospho-PAK5/6 (Ser602/Ser560)
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 81kD,75kD(Calculated).
蛋白号: Q9P286 | Q9NQU5
RRID: AB_2846836

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-PAK5/6 (Ser602/Ser560) Antibody detects endogenous levels of PAK5/6 only when phosphorylated at Ser602/560.
RRID:
AB_2846836
引用格式: Affinity Biosciences Cat# AF3522, RRID:AB_2846836.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDKN1A activated kinase 6; EC 2.7.1.37; p21 activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A) activated kinase 6; p21-activated kinase 6; p21activated kinase 6; PAK 5; PAK 6; PAK-5; PAK-6; PAK5; Pak6; PAK6_HUMAN; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; Serine/threonine-protein kinase PAK 6; EC 2.7.11.1; KIAA1264; MGC26232; p21 activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A) activated kinase 7; p21-activated kinase 5; p21-activated kinase 7; PAK 5; PAK 7; PAK-5; PAK-7; PAK5; PAK7; PAK7_HUMAN; Protein kinase PAK5; Serine/threonine protein kinase PAK 7; Serine/threonine-protein kinase PAK 7;

抗原和靶标

免疫原:

A synthesized peptide derived from human PAK5/6 around the phosphorylation site of Ser602/560.

Uniprot:

Q9P286(PAK5_HUMAN) >>Visit HPA database.

Q9NQU5(PAK6_HUMAN) >>Visit HPA database.

基因/基因ID:
PAK6(106821730) | PAK5(56924)
表达:
Q9P286 PAK5_HUMAN:

Predominantly expressed in brain.

Q9NQU5 PAK6_HUMAN:

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.

序列:
MFGKKKKKIEISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTIVRGNKPCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGASSHGPGHAEENGFITFSQYSSESDTTADYTTEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDFARFSADYHSHLDSLSKPSEYSDLKWEYQRASSSSPLDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQTSPQPTMRQRSRSGSGLQEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTMCIPKVDYDRAQMVLSPPLSGSDTYPRGPAKLPQSQSKSGYSSSSHQYPSGYHKATLYHHPSLQSSSQYISTASYLSSLSLSSSTYPPPSWGSSSDQQPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH

MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTVVRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPGPPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC

翻译修饰 - Q9P286/Q9NQU5 作为底物

Site PTM Type Enzyme
Y159 Phosphorylation
K167 Acetylation
Y182 Phosphorylation
S183 Phosphorylation
S226 Phosphorylation
S270 Phosphorylation
S271 Phosphorylation
Y272 Phosphorylation
S277 Phosphorylation
T281 Phosphorylation
R285 Methylation
S286 Phosphorylation
S290 Phosphorylation
S345 Phosphorylation
S347 Phosphorylation
T349 Phosphorylation
Y350 Phosphorylation
S573 Phosphorylation Q9P286 (PAK5)
S602 Phosphorylation
T606 Phosphorylation
Site PTM Type Enzyme
T69 Phosphorylation
Y81 Phosphorylation
S83 Phosphorylation
T99 Phosphorylation
S104 Phosphorylation
S113 Phosphorylation
Y129 Phosphorylation
S132 Phosphorylation
S135 Phosphorylation
Y144 Phosphorylation
S165 Phosphorylation Q16539 (MAPK14)
S179 Phosphorylation
S207 Phosphorylation
S246 Phosphorylation
S248 Phosphorylation
S308 Phosphorylation
T326 Phosphorylation
S327 Phosphorylation
S328 Phosphorylation
T339 Phosphorylation
S346 Phosphorylation
S347 Phosphorylation
S351 Phosphorylation
T354 Phosphorylation
S360 Phosphorylation
Y365 Phosphorylation
T371 Phosphorylation
K528 Ubiquitination
S560 Phosphorylation Q9NQU5 (PAK6)
T564 Phosphorylation P52564 (MAP2K6)
Y566 Phosphorylation P52564 (MAP2K6)
S616 Phosphorylation

翻译修饰 - Q9P286/Q9NQU5 作为激酶

Substrate Site Source
P15923 (TCF3) S39 Uniprot
P15976 (GATA1) S161 Uniprot
P15976 (GATA1) S187 Uniprot
Q9P286 (PAK5) S573 Uniprot
Substrate Site Source
P10275 (AR) S579 Uniprot
P53667 (LIMK1) T508 Uniprot
Q00987 (MDM2) T158 Uniprot
Q00987 (MDM2) S186 Uniprot
Q92934 (BAD) S75 Uniprot
Q9BY11 (PACSIN1) S346 Uniprot
Q9NQU5 (PAK6) S560 Uniprot

研究背景

功能:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

翻译修饰:

Autophosphorylated when activated by CDC42/p21.

细胞定位:

Mitochondrion. Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Predominantly expressed in brain.

亚基结构:

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH.

蛋白家族:

An autoinhibitory domain is present in the N-terminal region of the protein.

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

功能:

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.

翻译修饰:

Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.

细胞定位:

Cytoplasm. Nucleus.
Note: Cotranslocates into nucleus with AR in response to androgen induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.

亚基结构:

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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