| 产品: | 磷酸化 hFANCI (Ser730) 抗体 |
| 货号: | AF3527 |
| 描述: | Rabbit polyclonal antibody to Phospho-hFANCI (Ser730) |
| 应用: | ELISA(peptide) |
| 反应: | Human, Mouse |
| 分子量: | 150kD; 149kD(Calculated). |
| 蛋白号: | Q9NVI1 |
| RRID: | AB_2846841 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3527, RRID:AB_2846841.
展开/折叠
FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia, complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI;
抗原和靶标
A synthesized peptide derived from human hFANCI around the phosphorylation site of Ser730.
- Q9NVI1 FANCI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK
翻译修饰 - Q9NVI1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S559 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S565 | Phosphorylation | Uniprot | |
| Y606 | Phosphorylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K646 | Acetylation | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K780 | Ubiquitination | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| K799 | Ubiquitination | Uniprot | |
| T800 | Phosphorylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| K926 | Ubiquitination | Uniprot | |
| K939 | Ubiquitination | Uniprot | |
| T952 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1000 | Phosphorylation | Uniprot | |
| K1015 | Ubiquitination | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| K1040 | Ubiquitination | Uniprot | |
| K1068 | Ubiquitination | Uniprot | |
| K1105 | Ubiquitination | Uniprot | |
| K1107 | Ubiquitination | Uniprot | |
| S1120 | Phosphorylation | Uniprot | |
| S1121 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K1196 | Ubiquitination | Uniprot | |
| K1199 | Ubiquitination | Uniprot | |
| K1221 | Ubiquitination | Uniprot | |
| K1229 | Ubiquitination | Uniprot | |
| K1232 | Ubiquitination | Uniprot | |
| T1238 | Phosphorylation | Uniprot | |
| K1248 | Ubiquitination | Uniprot | |
| K1272 | Ubiquitination | Uniprot | |
| K1280 | Ubiquitination | Uniprot | |
| K1290 | Ubiquitination | Uniprot | |
| S1312 | Phosphorylation | Uniprot |
研究背景
Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.
Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding.
Phosphorylated in response to DNA damage by ATM and/or ATR.
Nucleus.
Note: Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.
Interacts with FANCD2; the interaction is direct. Interacts with FANCL. Interacts with MTMR15/FAN1. Interacts with POLN.
The C-terminal 30 residues are probably required for function in DNA repair.
研究领域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.