| 产品: | 磷酸化 Nanog (Thr280) 抗体 |
| 货号: | AF3535 |
| 描述: | Rabbit polyclonal antibody to Phospho-Nanog (Thr280) |
| 应用: | IHC IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 35kD(Calculated). |
| 蛋白号: | Q9H9S0 |
| RRID: | AB_2846849 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3535, RRID:AB_2846849.
展开/折叠
Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox; NANOG_HUMAN;
抗原和靶标
A synthesized peptide derived from human Nanog around the phosphorylation site of Thr280.
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
- Q9H9S0 NANOG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV
翻译修饰 - Q9H9S0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| Y35 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| T50 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S56 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) | Uniprot |
| T78 | Phosphorylation | Q02156 (PRKCE) , P28482 (MAPK1) | Uniprot |
| S79 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| S135 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| Y174 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| T200 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| S258 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| T286 | Phosphorylation | Uniprot | |
| T289 | Phosphorylation | Uniprot |
研究背景
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Nucleus.
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1. Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity).
Belongs to the Nanog homeobox family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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