| 产品: | 磷酸化 WNK1 (Thr60) 抗体 |
| 货号: | AF3536 |
| 描述: | Rabbit polyclonal antibody to Phospho-WNK1 (Thr60) |
| 应用: | IHC IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 250kD; 251kD(Calculated). |
| 蛋白号: | Q9H4A3 |
| RRID: | AB_2846850 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3536, RRID:AB_2846850.
展开/折叠
Erythrocyte 65 kDa protein; HSAN2; HSN2; hWNK1; KDP; KIAA0344; Kinase deficient protein; MGC163339; MGC163341; p65; PPP1R167; PRKWNK1; Prostate derived sterile 20 like kinase; Protein kinase lysine deficient 1; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; Protein phosphatase 1, regulatory subunit 167; PSK; Serine/threonine protein kinase WNK1 1; Serine/threonine protein kinase WNK1 2; Serine/threonine protein kinase WNK1; Serine/threonine-protein kinase WNK1; With no K; WNK lysine deficient protein kinase 1; WNK lysine deficient protein kinase 1 isoform; WNK1; WNK1_HUMAN;
抗原和靶标
A synthesized peptide derived from human WNK1 around the phosphorylation site of Thr60.
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
- Q9H4A3 WNK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT
翻译修饰 - Q9H4A3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) , O75582 (RPS6KA5) | Uniprot |
| R66 | Methylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| S378 | Phosphorylation | Q9H4A3 (WNK1) | Uniprot |
| S382 | Phosphorylation | Q9BYP7 (WNK3) , Q9Y3S1 (WNK2) , Q9H4A3 (WNK1) | Uniprot |
| S599 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| T1098 | Phosphorylation | Uniprot | |
| Y1102 | Phosphorylation | Uniprot | |
| K1104 | Acetylation | Uniprot | |
| S1105 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot | |
| S1110 | Phosphorylation | Uniprot | |
| T1115 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | Uniprot | |
| K1119 | Acetylation | Uniprot | |
| K1128 | Acetylation | Uniprot | |
| Y1215 | Phosphorylation | Uniprot | |
| S1220 | Phosphorylation | Uniprot | |
| S1253 | Phosphorylation | Uniprot | |
| S1261 | Phosphorylation | Uniprot | |
| S1285 | Phosphorylation | Uniprot | |
| S1295 | Phosphorylation | Uniprot | |
| S1297 | Phosphorylation | Uniprot | |
| S1300 | Phosphorylation | Uniprot | |
| K1653 | Ubiquitination | Uniprot | |
| K1658 | Ubiquitination | Uniprot | |
| T1810 | Phosphorylation | Uniprot | |
| T1848 | Phosphorylation | Uniprot | |
| S1849 | Phosphorylation | Uniprot | |
| S1887 | Phosphorylation | Uniprot | |
| S1935 | Phosphorylation | Uniprot | |
| S1959 | Phosphorylation | Uniprot | |
| S1961 | Phosphorylation | Uniprot | |
| T1963 | Phosphorylation | Uniprot | |
| T1968 | Phosphorylation | Uniprot | |
| T1970 | Phosphorylation | Uniprot | |
| S1978 | Phosphorylation | Uniprot | |
| S2002 | Phosphorylation | Uniprot | |
| S2005 | Phosphorylation | Uniprot | |
| S2011 | Phosphorylation | Uniprot | |
| S2012 | Phosphorylation | Uniprot | |
| S2027 | Phosphorylation | Uniprot | |
| S2029 | Phosphorylation | Uniprot | |
| S2032 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S2037 | Phosphorylation | Uniprot | |
| S2038 | Phosphorylation | Uniprot | |
| S2065 | Phosphorylation | Uniprot | |
| Y2102 | Phosphorylation | Uniprot | |
| S2121 | Phosphorylation | Uniprot | |
| T2128 | Phosphorylation | Uniprot | |
| S2145 | Phosphorylation | Uniprot | |
| K2243 | Ubiquitination | Uniprot | |
| T2245 | Phosphorylation | Uniprot | |
| T2247 | Phosphorylation | Uniprot | |
| S2265 | Phosphorylation | Uniprot | |
| S2270 | Phosphorylation | Uniprot | |
| Y2276 | Phosphorylation | Uniprot | |
| S2286 | Phosphorylation | Uniprot | |
| S2294 | Phosphorylation | Uniprot | |
| S2370 | Phosphorylation | Uniprot | |
| S2372 | Phosphorylation | Uniprot | |
| R2380 | Methylation | Uniprot |
翻译修饰 - Q9H4A3 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O95747 (OXSR1) | T185 | Uniprot |
| O95747 (OXSR1) | S325 | Uniprot |
| Q15796 (SMAD2) | S110 | Uniprot |
| Q15796 (SMAD2) | S260 | Uniprot |
| Q15796 (SMAD2) | S465 | Uniprot |
| Q8N9I0 (SYT2) | T199 | Uniprot |
| Q8N9I0 (SYT2) | T383 | Uniprot |
| Q9H4A3 (WNK1) | S378 | Uniprot |
| Q9H4A3 (WNK1) | S382 | Uniprot |
| Q9UEW8 (STK39) | T231 | Uniprot |
| Q9UEW8 (STK39) | S371 | Uniprot |
| Q9UHW9 (SLC12A6) | T991 | Uniprot |
| Q9UHW9 (SLC12A6) | T1048 | Uniprot |
研究背景
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity).
Dominant-negative regulator of the longer isoform 1. Does not have kinase activity, does not directly inhibit WNK4 and has no direct effect on sodium and chloride ion transport. Downregulates sodium-chloride cotransporter activity indirectly by inhibiting isoform 1, it associates with isoform 1 and attenuates its kinase activity. In kidney, may play an important role regulating sodium and potassium balance.
Autophosphorylation at Ser-382 is inhibited by intracellular calcium.
May be O-glycosylated.
Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
Cytoplasm.
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
Interacts with SYT2 (By similarity). Interacts with WNK3 and WNK4 (By similarity). Interacts with KLHL3. Isoform 3: Interacts with isoform 1 (By similarity).
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
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