产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3545, RRID:AB_2846859.
展开/折叠
M3K3_HUMAN; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; Mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3;
抗原和靶标
A synthesized peptide derived from human MAP3K3 around the phosphorylation site of Ser166.
- Q99759 M3K3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEQEALNSIMNDLVALQMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY
翻译修饰 - Q99759 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K102 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | O00141 (SGK1) | Uniprot |
| S168 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| Y275 | Phosphorylation | Uniprot | |
| Y288 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | O00141 (SGK1) | Uniprot |
| S340 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| T516 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Q99759 (MAP3K3) | Uniprot |
| T528 | Phosphorylation | Uniprot | |
| T530 | Phosphorylation | Uniprot |
翻译修饰 - Q99759 作为激酶
研究背景
Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.
Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
限制条款
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