产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3598, RRID:AB_2846912.
展开/折叠
;Dual specificity tyrosine phosphorylation regulated kinase; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A; Dual specificity tyrosine phosphorylation regulated kinase 1; Dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK 1 related kinase; Dual specificity YAK1 related kinase; Dual specificity YAK1-related kinase; DYR1A_HUMAN; DYRK 1; DYRK 1A; DYRK; DYRK1; Dyrk1a; DYRKA; hMNB; HP 86; HP86; Minibrain (Drosophila) homolog; Minibrain homolog; Minibrain, Drosophila, homolog of; MNB; MNB protein kinase; Mnb protein kinase homolog hp86; MNB protein kinase, Serine/threonine-specific; MNB/DYRK protein kinase; MNBH; MRD7; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; Protein kinase minibrain homolog; Serine/threonine kinase MNB;
抗原和靶标
A synthesized peptide derived from human DYR1A around the phosphorylation site of Tyr449.
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
- Q13627 DYR1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS
翻译修饰 - Q13627 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| Y104 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K105 | Acetylation | Uniprot | |
| Y111 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y112 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y145 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| Y159 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K167 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K304 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y319 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y321 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| S324 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| Y449 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| K453 | Ubiquitination | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| T532 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| T553 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| Y705 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot |
翻译修饰 - Q13627 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O00401 (WASL) | T199 | Uniprot |
| O00401 (WASL) | T205 | Uniprot |
| O00401 (WASL) | T262 | Uniprot |
| O43524 (FOXO3) | S330 | Uniprot |
| O43597 (SPRY2) | T75 | Uniprot |
| O75533 (SF3B1) | T273 | Uniprot |
| O75533 (SF3B1) | T434 | Uniprot |
| P01106 (MYC) | S62 | Uniprot |
| P04637 (TP53) | S15 | Uniprot |
| P10636-8 (MAPT) | S199 | Uniprot |
| P10636-8 (MAPT) | S202 | Uniprot |
| P10636-8 (MAPT) | T205 | Uniprot |
| P10636-8 (MAPT) | T212 | Uniprot |
| P10636-8 (MAPT) | T217 | Uniprot |
| P10636 (MAPT) | S369 | Uniprot |
| P10636-8 (MAPT) | S396 | Uniprot |
| P10636-8 (MAPT) | S404 | Uniprot |
| P10636-8 (MAPT) | S422 | Uniprot |
| P10636 (MAPT) | S519 | Uniprot |
| P10636 (MAPT) | T529 | Uniprot |
| P10636 (MAPT) | S721 | Uniprot |
| P16220 (CREB1) | S133 | Uniprot |
| P24385 (CCND1) | T286 | Uniprot |
| P24928 (POLR2A) | S1616 | Uniprot |
| P24928 (POLR2A) | S1619 | Uniprot |
| P37840 (SNCA) | S87 | Uniprot |
| P46821 (MAP1B) | S1400 | Uniprot |
| P49418 (AMPH) | S262 | Uniprot |
| P49418 (AMPH) | S272 | Uniprot |
| P49418 (AMPH) | S276 | Uniprot |
| P49418 (AMPH) | S285 | Uniprot |
| P49418 (AMPH) | S293 | Uniprot |
| P49418 (AMPH) | S295 | Uniprot |
| P49418 (AMPH) | T310 | Uniprot |
| P49418 (AMPH) | T312 | Uniprot |
| P49768 (PSEN1) | T354 | Uniprot |
| P53805 (RCAN1) | S167 | Uniprot |
| P53805 (RCAN1) | T247 | Uniprot |
| P55211 (CASP9) | T125 | Uniprot |
| P61764 (STXBP1) | T479 | Uniprot |
| Q05193 (DNM1) | S795 | Uniprot |
| Q05193 (DNM1) | S857 | Uniprot |
| Q07955 (SRSF1) | S227 | Uniprot |
| Q07955 (SRSF1) | S234 | Uniprot |
| Q07955 (SRSF1) | S238 | Uniprot |
| Q12778 (FOXO1) | S329 | Uniprot |
| Q13627 (DYRK1A) | Y104 | Uniprot |
| Q13627 (DYRK1A) | Y111 | Uniprot |
| Q13627 (DYRK1A) | Y140 | Uniprot |
| Q13627 (DYRK1A) | Y159 | Uniprot |
| Q13627 (DYRK1A) | Y177 | Uniprot |
| Q13627 (DYRK1A) | S310 | Uniprot |
| Q13627 (DYRK1A) | Y319 | Uniprot |
| Q13627 (DYRK1A) | Y321 | Uniprot |
| Q13627 (DYRK1A) | T402 | Uniprot |
| Q13627 (DYRK1A) | Y449 | Uniprot |
| Q13627-1 (DYRK1A) | S519 | Uniprot |
| Q13627-2 (DYRK1A) | S520 | Uniprot |
| Q13627 (DYRK1A) | S529 | Uniprot |
| Q49AN0 (CRY2) | S558 | Uniprot |
| Q52LA3 (LIN52) | S28 | Uniprot |
| Q96EB6 (SIRT1) | T530 | Uniprot |
| Q96S94 (CCNL2) | S330 | Uniprot |
| Q96S94-1 (CCNL2) | S338 | Uniprot |
| Q96S94-1 (CCNL2) | S369 | Uniprot |
研究背景
Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).
Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).
Nucleus. Nucleus speckle.
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
Interacts RAD54L2/ARIP4 (By similarity). Interacts with CRY2 (By similarity). Interacts with RANBP9. Interacts with WDR68. Interacts with SRSF6. Interacts with SIRT1 (By similarity).
(Microbial infection) Interacts with human adenovirus 5 E1A protein.
The polyhistidine repeats act as targeting signals to nuclear speckles.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
限制条款
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