产品: 磷酸化 4E-BP1 (Ser65) 抗体
货号: AF3601
描述: Rabbit polyclonal antibody to Phospho-4E-BP1 (Ser65)
应用: ELISA(peptide)
反应: Human, Mouse, Rat
分子量: 13kD(Calculated).
蛋白号: Q13541
RRID: AB_2846915

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产品描述

来源:
Rabbit
应用:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Ser65.
RRID:
AB_2846915
引用格式: Affinity Biosciences Cat# AF3601, RRID:AB_2846915.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Ser65.

Uniprot:
基因/基因ID:
序列:
MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI

翻译修饰 - Q13541 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S5 Phosphorylation
S6 Phosphorylation
S8 Phosphorylation Q13315 (ATM)
T10 Phosphorylation
S12 Phosphorylation
Y34 Phosphorylation
S35 Phosphorylation
T36 Phosphorylation P51449 (RORC) , O75582 (RPS6KA5) , P31751 (AKT2) , P42345 (MTOR)
T37 Phosphorylation P49841 (GSK3B) , Q16539 (MAPK14) , P31749 (AKT1) , P42345 (MTOR) , P06493 (CDK1) , P28482 (MAPK1) , Q5S007 (LRRK2)
T41 Phosphorylation P42345 (MTOR) , P49674 (CSNK1E)
S44 Phosphorylation P42345 (MTOR)
T45 Phosphorylation O75582 (RPS6KA5) , P28482 (MAPK1) , P42345 (MTOR) , P31751 (AKT2) , P51449 (RORC)
T46 Phosphorylation P06493 (CDK1) , Q5S007 (LRRK2) , Q16539 (MAPK14) , P42345 (MTOR) , P31749 (AKT1) , P28482 (MAPK1) , P49841 (GSK3B)
T50 Phosphorylation P49674 (CSNK1E)
Y54 Phosphorylation
K57 Ubiquitination
C62 S-Nitrosylation
R63 Methylation
S65 Phosphorylation P27361 (MAPK3) , P42345 (MTOR) , P28482 (MAPK1) , O75582 (RPS6KA5) , Q8TAI7 (RHEBL1) , P31749 (AKT1) , Q92630 (DYRK2) , Q9P1W9 (PIM2) , P06493 (CDK1) , Q16539 (MAPK14)
T68 Phosphorylation
K69 Ubiquitination
T70 Phosphorylation P28482 (MAPK1) , P06493 (CDK1) , Q16539 (MAPK14) , P42345 (MTOR) , P41279 (MAP3K8)
T77 Phosphorylation
T82 Phosphorylation
S83 Phosphorylation P42345 (MTOR) , P06493 (CDK1) , P28482 (MAPK1)
S85 Phosphorylation
S86 Phosphorylation
S94 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
S96 Phosphorylation
S101 Phosphorylation Q92630 (DYRK2) , P42345 (MTOR)
S112 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1) , Q13315 (ATM)

研究背景

功能:

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

翻译修饰:

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.

Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

亚基结构:

Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts (via TOS motif) with RPTOR; promoting phosphorylation by mTORC1.

蛋白家族:

The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex.

Belongs to the eIF4E-binding protein family.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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