产品: 磷酸化 SLP76 (Tyr128) 抗体
货号: AF3607
描述: Rabbit polyclonal antibody to Phospho-SLP76 (Tyr128)
应用: ELISA(peptide)
反应: Human, Mouse, Rat
分子量: 65~85kD; 60kD(Calculated).
蛋白号: Q13094
RRID: AB_2846921

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产品描述

来源:
Rabbit
应用:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-SLP76 (Tyr128) Antibody detects endogenous levels of SLP76 only when phosphorylated at Tyr128.
RRID:
AB_2846921
引用格式: Affinity Biosciences Cat# AF3607, RRID:AB_2846921.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte cytosolic protein 2; SH2 domain containing leukocyte protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein;

抗原和靶标

免疫原:

A synthesized peptide derived from human SLP76 around the phosphorylation site of Tyr128.

Uniprot:
基因/基因ID:
表达:
Q13094 LCP2_HUMAN:

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

序列:
MALRNVPFRSEVLGWDPDSLADYFKKLNYKDCEKAVKKYHIDGARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGEDDGDYESPNEEEEAPVEDDADYEPPPSNDEEALQNSILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP

翻译修饰 - Q13094 作为底物

Site PTM Type Enzyme
Y29 Phosphorylation
K30 Ubiquitination
Y39 Phosphorylation
S106 Phosphorylation
S107 Phosphorylation
Y113 Phosphorylation P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK)
Y128 Phosphorylation P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK)
S130 Phosphorylation
Y145 Phosphorylation P43403 (ZAP70) , P06239 (LCK) , P42681 (TXK)
S169 Phosphorylation
S171 Phosphorylation
Y173 Phosphorylation Q08881 (ITK)
S207 Phosphorylation
S220 Phosphorylation
S222 Phosphorylation
T227 Phosphorylation
S234 Phosphorylation
S246 Phosphorylation
K260 Ubiquitination
S264 Phosphorylation
S268 Phosphorylation
R273 Methylation
S274 Phosphorylation
S298 Phosphorylation
S339 Phosphorylation
T341 Phosphorylation
S344 Phosphorylation
S374 Phosphorylation
S376 Phosphorylation Q92918 (MAP4K1)
R392 Methylation
S410 Phosphorylation
S417 Phosphorylation
Y423 Phosphorylation P06239 (LCK) , P43403 (ZAP70)
S425 Phosphorylation
Y426 Phosphorylation P06239 (LCK) , P43403 (ZAP70)
T428 Phosphorylation
K438 Ubiquitination
T454 Phosphorylation
T455 Phosphorylation
T456 Phosphorylation
Y459 Phosphorylation
Y465 Phosphorylation
Y470 Phosphorylation
Y476 Phosphorylation
Y483 Phosphorylation
K492 Ubiquitination
K516 Ubiquitination
Y522 Phosphorylation
Y532 Phosphorylation

研究背景

功能:

Involved in T-cell antigen receptor mediated signaling.

翻译修饰:

Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

亚基结构:

Interacts with SLA. Interacts with CBLB (By similarity). Interacts with GRB2. Interacts with SHB. Interacts with PRAM1. Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus). Interacts with FYB1 and the phosphorylated form of FYB2.

蛋白家族:

The SH2 domain mediates interaction with phosphorylated CD6 (PubMed:16914752). The SH2 domain mediates interaction with SHB (PubMed:12084069).

研究领域

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

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