| 产品: | 磷酸化 CDC16/APC6 (Ser560) 抗体 |
| 货号: | AF3608 |
| 描述: | Rabbit polyclonal antibody to Phospho-CDC16/APC6 (Ser560) |
| 应用: | IHC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 72kD(Calculated). |
| 蛋白号: | Q13042 |
| RRID: | AB_2846922 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3608, RRID:AB_2846922.
展开/折叠
ANAPC6; Anaphase promoting complex subunit 6; Anaphase-promoting complex subunit 6; Apc 6; APC6; CDC 16; CDC16 (cell division cycle 16 S. cerevisiae homolog); Cdc16; CDC16 homolog; CDC16 protein; CDC16_HUMAN; CDC16Hs; Cell division cycle 16; Cell division cycle 16 homolog; Cell division cycle protein 16 homolog; CUT9; Cyclosome subunit 6;
抗原和靶标
A synthesized peptide derived from human CDC16/APC6 around the phosphorylation site of Ser560.
- Q13042 CDC16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHALRSRKLDKLYEACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSDWEMSQSSIKSSICLLRGKIYDALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLESLPLSKLCNEEQELLRFLFENKLKKYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNKANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEHDQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKTAEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHSLMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIISPPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST
翻译修饰 - Q13042 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T50 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | P06493 (CDK1) , P53350 (PLK1) | Uniprot |
| K115 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| T420 | Phosphorylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| S560 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K572 | Ubiquitination | Uniprot | |
| T581 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S586 | Phosphorylation | Uniprot | |
| T589 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| S607 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot |
研究背景
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
V-shaped homodimer. The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. Interacts with PPP5C and CDC20. Interacts with CDC26.
TPR repeats 1-7 mediate homodimerization, while the C-terminal TPR repeats bind to CDC26, burying its hydrophobic N-terminus.
Belongs to the APC6/CDC16 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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