| 产品: | 磷酸化 MLK1/2 (Thr312/Thr266) 抗体 |
| 货号: | AF3630 |
| 描述: | Rabbit polyclonal antibody to Phospho-MLK1/2 (Thr312/Thr266) |
| 应用: | ELISA(peptide) |
| 反应: | Human, Mouse, Rat |
| 分子量: | 122kD,104kD(Calculated). |
| 蛋白号: | P80192 | Q02779 |
| RRID: | AB_2846944 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3630, RRID:AB_2846944.
展开/折叠
M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; MLK1; PRKE1; M3K10_HUMAN; MAP3K 10; Map3k10; MEKK10; Mitogen activated protein kinase kinase kinase 10; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 derived nonreceptor type serine/threonine kinase; MKN28 kinase; MLK 2; MST; Protein kinase MST;
抗原和靶标
A synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266.
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Q02779 M3K10_HUMAN:Expressed in brain and skeletal muscle.
- P80192 M3K9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPSRALLGCLASAAAAAPPGEDGAGAGAEEEEEEEEEAAAAVGPGELGCDAPLPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYVTPRSAFSSRCQPGGEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTNILDQLTTIEESGFFEMPKDSFHCLQDNWKHEIQEMFDQLRAKEKELRTWEEELTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKSRLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLINSRSSPPASPTIIPRLRAIQLTPGESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELASGDEGSPQRREKANGLSTPSESPHFHLGLKSLVDGYKQWSSSAPNLVKGPRSSPALPGFTSLMEMEDEDSEGPGSGESRLQHSPSQSYLCIPFPRGEDGDGPSSDGIHEEPTPVNSATSTPQLTPTNSLKRGGAHHRRCEVALLGCGAVLAATGLGFDLLEAGKCQLLPLEEPEPPAREEKKRREGLFQRSSRPRRSTSPPSRKLFKKEEPMLLLGDPSASLTLLSLSSISECNSTRSLLRSDSDEIVVYEMPVSPVEAPPLSPCTHNPLVNVRVERFKRDPNQSLTPTHVTLTTPSQPSSHRRTPSDGALKPETLLASRSPSSNGLSPSPGAGMLKTPSPSRDPGEFPRLPDPNVVFPPTPRRWNTQQDSTLERPKTLEFLPRPRPSANRQRLDPWWFVSPSHARSTSPANSSSTETPSNLDSCFASSSSTVEERPGLPALLPFQAGPLPPTERTLLDLDAEGQSQDSTVPLCRAELNTHRPAPYEIQQEFWS
- Q02779 M3K10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGVFPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDFGSILKRLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLRRREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGFEHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHTPIAPGFASLNEMEEFAEAEDGGSSVPPSPYSTPSYLSVPLPAEPSPGARAPWEPTPSAPPARWGHGARRRCDLALLGCATLLGAVGLGADVAEARAADGEEQRRWLDGLFFPRAGRFPRGLSPPARPHGRREDVGPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAAPAAPSPPPSPPAPTPTPSPSTNPLVDLELESFKKDPRQSLTPTHVTAACAVSRGHRRTPSDGALGQRGPPEPAGHGPGPRDLLDFPRLPDPQALFPARRRPPEFPGRPTTLTFAPRPRPAASRPRLDPWKLVSFGRTLTISPPSRPDTPESPGPPSVQPTLLDMDMEGQNQDSTVPLCGAHGSH
翻译修饰 - P80192/Q02779 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y110 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| T305 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| S308 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| T312 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| T452 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| T535 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| T554 | Phosphorylation | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| K594 | Acetylation | Uniprot | |
| K595 | Acetylation | Uniprot | |
| K607 | Acetylation | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| S693 | Phosphorylation | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| S807 | Phosphorylation | Uniprot | |
| T808 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T915 | Phosphorylation | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| T948 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S225 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| T558 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S830 | Phosphorylation | Uniprot |
翻译修饰 - P80192/Q02779 作为激酶
研究背景
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Homodimer.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Activates the JUN N-terminal pathway.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Expressed in brain and skeletal muscle.
Homodimer. Interacts with SH3RF2.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
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