产品: 磷酸化 SRPK2 (Ser494/Ser497) 抗体
货号: AF3633
描述: Rabbit polyclonal antibody to Phospho-SRPK2 (Ser494/Ser497)
应用: IHC
反应: Human, Mouse, Rat
分子量: 78kD,110~130kD; 78kD(Calculated).
蛋白号: P78362
RRID: AB_2846947

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-SRPK2 (Ser494/Ser497) Antibody detects endogenous levels of SRPK2 only when phosphorylated at Ser494/497.
RRID:
AB_2846947
引用格式: Affinity Biosciences Cat# AF3633, RRID:AB_2846947.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Human serine kinase SRPK2 mRNA, complete cds; Serine kinase SRPK2; Serine/arginine rich protein specific kinase 2; Serine/arginine-rich protein-specific kinase 2; Serine/threonine protein kinase SRPK2; SFRS protein kinase 2; SFRSK2; SR protein specific kinase 2; SR-protein-specific kinase 2; SRPK2; SRPK2_HUMAN; SRSF protein kinase 2 C-terminal;

抗原和靶标

免疫原:

A synthesized peptide derived from human SRPK2 around the phosphorylation site of Ser494/497.

Uniprot:
基因/基因ID:
表达:
P78362 SRPK2_HUMAN:

Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.

序列:
MSVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPPPLPDPTPPEPEEEILGSDDEEQEDPADYCKGGYHPVKIGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAELLEKRLQEIEELEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS

翻译修饰 - P78362 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
S52 Phosphorylation
K110 Ubiquitination
K127 Ubiquitination
K130 Ubiquitination
K179 Ubiquitination
Y182 Phosphorylation
K216 Ubiquitination
K241 Ubiquitination
S250 Phosphorylation
K285 Ubiquitination
K300 Ubiquitination
S312 Phosphorylation
Y319 Phosphorylation
C320 S-Nitrosylation
K326 Ubiquitination
T328 Phosphorylation
T376 Phosphorylation
S380 Phosphorylation
S423 Phosphorylation
Y427 Phosphorylation
S483 Phosphorylation
S484 Phosphorylation
T492 Phosphorylation P31749 (AKT1)
S494 Phosphorylation
S496 Phosphorylation
S497 Phosphorylation
T498 Phosphorylation
K503 Ubiquitination
K527 Ubiquitination
K539 Acetylation
K539 Ubiquitination
T547 Phosphorylation
R548 Methylation
S608 Phosphorylation
S616 Phosphorylation
K618 Ubiquitination
K637 Ubiquitination

翻译修饰 - P78362 作为激酶

Substrate Site Source
Q9UKV3 (ACIN1) S1180 Uniprot

研究背景

功能:

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

翻译修饰:

Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.

Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.

细胞定位:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.

亚基结构:

Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs).

蛋白家族:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.

文献引用

1). Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022 (PubMed: 35054902) [IF=5.6]

Application: IF/ICC    Species: Mice    Sample:

Figure 7 Double-labeling immunofluorescence and confocal microscopy to p38-P Thr180/Tyr182 or SAPK/JNK-PThr183/Thr185 (green), and AT8 (red) in inoculated hTau at the age of six months and killed at the age of nine months shows co-localization of the active kinases with phospho-tau deposition (white arrows) (A and B, respectively for p38-P and SAPK-JNK-P) in dentate gyrus neurons. Double-labelling immunofluorescence and confocal microscopy to PKAα/β-P Tyr197 and AT8 in inoculated hTau mice shows cytoplasmic granular PKAα/β-P Tyr197 granules in CA1 neurons with abnormal phospho-tau deposits in neurons (C, arrow). Cytoplasmic SRPK2-Ser497 immunoreactivity is found in neurons with phospho-tau deposits in inoculated hTau mice (D). Paraffin sections, nuclei are stained with DRAQ5™ (blue), A, B, bar = 10 µm, C and D, bar = 20 µm. CLK1 immunoreactivity in the CA1 region of non-inoculated (E) and inoculated hTau with AD sarkosyl-insoluble fractions (F); small cytoplasmic granules are seen in inoculated hTau. Paraffin sections, slightly counterstained with haematoxylin, bar = 40 µm.

2). Dysregulated Protein Phosphorylation in a Mouse Model of FTLD-Tau. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2022 (PubMed: 35848963) [IF=3.2]

3). Dysregulated protein phosphorylation in tauopathy. Research Square, 2022

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