Phospho-FOXL2 (Ser263) Antibody - #AF3649

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产品: 磷酸化 FOXL2 (Ser263) 抗体
货号: AF3649
描述: Rabbit polyclonal antibody to Phospho-FOXL2 (Ser263)
应用: IF/ICC
反应: Human, Mouse, Rat
分子量: 39kD(Calculated).
蛋白号: P58012
RRID: AB_2846963

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 200ul RMB¥ 3800 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-FOXL2 (Ser263) Antibody detects endogenous levels of FOXL2 only when phosphorylated at Ser263.
RRID:
AB_2846963
引用格式: Affinity Biosciences Cat# AF3649, RRID:AB_2846963.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Blepharophimosis; Blepharophimosis epicanthus inversus and ptosis 1; Blepharophimosis epicanthus inversus and ptosis; BPES 1; BPES; BPES1; Epicanthus inversus and ptosis 1; Forkhead box L2; Forkhead box protein L2; Forkhead transcription factor FOXL2; FOX L2; FOXL 2; FOXL2; FOXL2_HUMAN; PFRK; PINTO; PITUITARY FORKHEAD FACTOR; POF 3; POF3;

抗原和靶标

免疫原:

A synthesized peptide derived from human FOXL2 around the phosphorylation site of Ser263.

Uniprot:

P58012(FOXL2_HUMAN) >>Visit HPA database.

基因/基因ID:
FOXL2(668)
表达:
P58012 FOXL2_HUMAN:

In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.

序列:
MMASYPEPEDAAGALLAPETGRTVKEPEGPPPSPGKGGGGGGGTAPEKPDPAQKPPYSYVALIAMAIRESAEKRLTLSGIYQYIIAKFPFYEKNKKGWQNSIRHNLSLNECFIKVPREGGGERKGNYWTLDPACEDMFEKGNYRRRRRMKRPFRPPPAHFQPGKGLFGAGGAAGGCGVAGAGADGYGYLAPPKYLQSGFLNNSWPLPQPPSPMPYASCQMAAAAAAAAAAAAAAGPGSPGAAAVVKGLAGPAASYGPYTRVQSMALPPGVVNSYNGLGGPPAAPPPPPHPHPHPHAHHLHAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQPELAMMHCSYWDHDSKTGALHSRLDL

翻译修饰 - P58012 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
Y5 Phosphorylation
K25 Sumoylation
S33 Phosphorylation P49841 (GSK3B)
Y81 Phosphorylation
Y83 Phosphorylation
Y91 Phosphorylation
K96 Acetylation
K96 Ubiquitination
S101 Phosphorylation
S107 Phosphorylation
K114 Sumoylation
Y127 Phosphorylation
K140 Acetylation
Y186 Phosphorylation
S211 Phosphorylation
Y215 Phosphorylation
S238 Phosphorylation
Y258 Phosphorylation
S263 Phosphorylation Q13153 (PAK1)
S323 Phosphorylation
S326 Phosphorylation
T329 Phosphorylation
K366 Acetylation

研究背景

功能:

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.

翻译修饰:

Sumoylated with SUMO1; sumoylation is required for transcriptional repression activity.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.

亚基结构:

Interacts with ESR1 (By similarity). Interacts with SMAD3 (By similarity). Interacts with DDX20. Interacts with UBE2I/UBC9.

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