| 产品: | 磷酸化 MeCP2 (Ser423) 抗体 |
| 货号: | AF3656 |
| 描述: | Rabbit polyclonal antibody to Phospho-MeCP2 (Ser423) |
| 应用: | IHC IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 52kD(Calculated). |
| 蛋白号: | P51608 |
| RRID: | AB_2846970 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3656, RRID:AB_2846970.
展开/折叠
AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;
抗原和靶标
A synthesized peptide derived from human MeCP2 around the phosphorylation site of Ser423.
- P51608 MECP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
翻译修饰 - P51608 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Q9H2X6 (HIPK2) | Uniprot |
| S86 | Phosphorylation | Uniprot | |
| R115 | Methylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K119 | Methylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| R162 | Methylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K210 | Methylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K267 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K321 | Acetylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K347 | Methylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| K449 | Acetylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot |
研究背景
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Present in all adult somatic tissues tested.
Interacts with FNBP3 (By similarity). Interacts with CDKL5. Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1. Interacts with TBL1X; recruits TBL1X to the heterochromatin foci.
限制条款
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