Phospho-XIRP1 (Ser295) Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from Rat heart, using Phospho-XIRP1 (Ser295) Antibody.

AF3762 at 1/100 staining Human esophageal cancer by IHC-P.

AF3762 at 1/100 staining Human ovarian cancer by IHC-P.

产品:	磷酸化 XIRP1 (Ser295) 抗体
货号:	AF3762
描述:	Rabbit polyclonal antibody to Phospho-XIRP1 (Ser295)
应用:	WB IHC
反应:	Human, Mouse, Rat
分子量:	110kDa; 123kD(Calculated).
蛋白号:	O70373
RRID:	AB_2847076

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2800	现货
200ul	RMB¥ 3800	现货

货期: 当天发货

联系销售

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Polyclonal

特异性:

Phospho-XIRP1 (Ser295) Antibody detects endogenous levels of XIRP1 only when phosphorylated at Ser295.

RRID:

AB_2847076
引用格式: Affinity Biosciences Cat# AF3762, RRID:AB_2847076.

偶联:

Unconjugated.

纯化:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

抗原和靶标

免疫原:

A synthesized peptide derived from mouse XIRP1 around the phosphorylation site of Ser295.

Uniprot:

O70373(XIRP1_MOUSE) >>Visit HPA database.

表达:

O70373 XIRP1_MOUSE:

Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level).

序列:

O70373 XIRP1_MOUSE:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADAQMQVAPTPTIQMRTEEDLSLPHPSAPEGLPPPPPKETFSKFQQQRQASELRRLYKHIHPELRKNLEEAVAEDLAEVLGSEEPTEGDVQCMRWIFENWRLDAIGDHERPAAREPVSGGNVQATSRKFEEGSFTNSSDQEPEGLRPSGGDVQAARQMFETKPLDALRGQEEATQTTMREPAATGDVQGTRKLFETRPLDRLGSRPSIQEQSPLELRSEIQELKGDVKKTVKLFQTEPLCAIQDAEGTIHEVKAACREEIQSNAVRSARWLFETRPLDAFNQDPSQVRVIRGISLEEGALPDVSATRWIFETQPLDAIREIEVDEKDFQPSPDLIPPGPDVQHQRHLFETCSLDTLKGERETEAEVPPKEEVIPGDVRSTLWLFETKPLDAFRDQVQVGHLQRVGHQEGEGLVTECLPSNGTSVLPLSQGVPQNDGLKGDVKTFKNLFETLPLDSIGQGEPSAYGNINRGQNTDSAEQSQGSDAPVYAMQDSRGQLHALTSVSREQVVGGDVQGYKWMFETQPLDTLGRSPSTIDVVRGITRQEVVAGDVGTTRWLFETQPLEMIHQQEQQKPEEEEGKGPGGPPPELPKKGDVQTIRWLFETYPMSELAEKRESEVTDPVSKAETQSCTWMFGPQSLNPAEGSGEQHLQTSQVPAGDRQTDRHVFETESLPASNQSSGRKPVRYCSRVEIPSGQVSRQKEVFQALEAGKKEVPETTINLGSIPTGSVHKFTWLFENCPMGSLAAESIRGDNLQEEQPKGSAGHGTPERQETAAERTLRTLHATPGILHHGGILMEARGPGELCLAKYVLPSPGQGRPYIRKEELVCGELPRIVRQVLRRTDVDQQGLLVQEDTAGQLQLHPLMLPGPGDPGNIEDMDPELQQLLACGLGVSVSKTGLVMQETGQGLVALTAYSLQPQLTSRAPERSSVQLLASCIDKGDLHSLHSLRWEPPTDPSSGPATEESQRVPPTESIIHVTPLDSTMEMGQLRISGSTPCPPPSRAAGKVVLPNGKPVAQAPLQEARKKRDISHAGQKGKAASGRPEASPLGSGAPDLQEAMQNLRLATAEAQSLHQQVLSRHPQGSDPVATSMPVQDVLQASTPATGVTQGSIRPVAGSEARIPAVPRKLL

研究背景

O70373_XIRP1_MOUSE

功能:

Protects actin filaments from depolymerization.

细胞定位:

Cell junction.
Note: Colocalizes with actin stress fibers.

组织特异性:

Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level).

亚基结构:

Interacts with FLNC and VASP (By similarity). Interacts with F-actin and CTNNB1.

蛋白家族:

Xin repeats bind F-actin.

Belongs to the Xin family.

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Phospho-XIRP1 (Ser295) Antibody - #AF3762