Phospho-JAK2 (Tyr1007/Tyr1008) Antibody - #AF3764
产品: | 磷酸化 JAK2 (Tyr1007/Tyr1008) 抗体 |
货号: | AF3764 |
描述: | Rabbit polyclonal antibody to Phospho-JAK2 (Tyr1007/Tyr1008) |
应用: | IHC |
反应: | Human, Mouse, Rat |
分子量: | 120~140kD; 131kD(Calculated). |
蛋白号: | O60674 |
RRID: | AB_2847078 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3764, RRID:AB_2847078.
展开/折叠
JAK 2; JAK-2; JAK2; JAK2_HUMAN; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase); Janus kinase 2; JTK 10; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2;
抗原和靶标
A synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007/1008.
- O60674 JAK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG
翻译修饰 - O60674 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y119 | Phosphorylation | O60674 (JAK2) | Uniprot |
K167 | Sumoylation | Uniprot | |
T174 | Phosphorylation | Uniprot | |
Y201 | Phosphorylation | O60674 (JAK2) | Uniprot |
Y206 | Phosphorylation | O60674 (JAK2) | Uniprot |
K207 | Ubiquitination | Uniprot | |
K212 | Ubiquitination | Uniprot | |
Y221 | Phosphorylation | O60674 (JAK2) | Uniprot |
K244 | Ubiquitination | Uniprot | |
K269 | Ubiquitination | Uniprot | |
Y317 | Phosphorylation | O60674 (JAK2) | Uniprot |
S358 | Phosphorylation | Uniprot | |
Y372 | Phosphorylation | Uniprot | |
Y373 | Phosphorylation | Uniprot | |
Y382 | Phosphorylation | Uniprot | |
Y423 | Phosphorylation | Uniprot | |
Y435 | Phosphorylation | Uniprot | |
S473 | Phosphorylation | Uniprot | |
S518 | Phosphorylation | Uniprot | |
T522 | Phosphorylation | Uniprot | |
S523 | Phosphorylation | P27361 (MAPK3) , O60674 (JAK2) | Uniprot |
Y570 | Phosphorylation | O60674 (JAK2) | Uniprot |
K581 | Ubiquitination | Uniprot | |
K630 | Sumoylation | Uniprot | |
Y637 | Phosphorylation | O60674 (JAK2) | Uniprot |
K728 | Ubiquitination | Uniprot | |
K762 | Ubiquitination | Uniprot | |
Y790 | Phosphorylation | Uniprot | |
Y813 | Phosphorylation | O60674 (JAK2) | Uniprot |
Y868 | Phosphorylation | O60674 (JAK2) | Uniprot |
K882 | Ubiquitination | Uniprot | |
K883 | Ubiquitination | Uniprot | |
K903 | Ubiquitination | Uniprot | |
S904 | Phosphorylation | Uniprot | |
K912 | Sumoylation | Uniprot | |
K914 | Sumoylation | Uniprot | |
Y931 | Phosphorylation | Uniprot | |
Y934 | Phosphorylation | Uniprot | |
S936 | Phosphorylation | Uniprot | |
Y940 | Phosphorylation | Uniprot | |
Y956 | Phosphorylation | Uniprot | |
Y966 | Phosphorylation | O60674 (JAK2) | Uniprot |
Y972 | Phosphorylation | O60674 (JAK2) | Uniprot |
K991 | Sumoylation | Uniprot | |
K999 | Ubiquitination | Uniprot | |
Y1007 | Phosphorylation | P40189 (IL6ST) , P00519 (ABL1) , O60674 (JAK2) | Uniprot |
Y1008 | Phosphorylation | O60674 (JAK2) , P40189 (IL6ST) | Uniprot |
K1011 | Sumoylation | Uniprot | |
K1011 | Ubiquitination | Uniprot | |
K1069 | Acetylation | Uniprot |
翻译修饰 - O60674 作为激酶
Substrate | Site | Source |
---|---|---|
O14543 (SOCS3) | Y204 | Uniprot |
O14543 (SOCS3) | Y221 | Uniprot |
O14744 (PRMT5) | Y297 | Uniprot |
O14744 (PRMT5) | Y304 | Uniprot |
O14744 (PRMT5) | Y307 | Uniprot |
O60674 (JAK2) | Y119 | Uniprot |
O60674 (JAK2) | Y201 | Uniprot |
O60674 (JAK2) | Y206 | Uniprot |
O60674 (JAK2) | Y221 | Uniprot |
O60674 (JAK2) | Y317 | Uniprot |
O60674 (JAK2) | S523 | Uniprot |
O60674 (JAK2) | Y570 | Uniprot |
O60674 (JAK2) | Y637 | Uniprot |
O60674 (JAK2) | Y813 | Uniprot |
O60674 (JAK2) | Y868 | Uniprot |
O60674 (JAK2) | Y966 | Uniprot |
O60674 (JAK2) | Y972 | Uniprot |
O60674 (JAK2) | Y1007 | Uniprot |
O60674 (JAK2) | Y1008 | Uniprot |
P00533-1 (EGFR) | Y1069 | Uniprot |
P00533 (EGFR) | Y1092 | Uniprot |
P04049-1 (RAF1) | Y340 | Uniprot |
P04049-1 (RAF1) | Y341 | Uniprot |
P10912 (GHR) | Y332 | Uniprot |
P10912 (GHR) | Y487 | Uniprot |
P16410 (CTLA4) | Y201 | Uniprot |
P19235-1 (EPOR) | Y368 | Uniprot |
P19235-1 (EPOR) | Y426 | Uniprot |
P19235-1 (EPOR) | Y454 | Uniprot |
P19235-1 (EPOR) | Y456 | Uniprot |
P19235-1 (EPOR) | Y468 | Uniprot |
P19235-1 (EPOR) | Y485 | Uniprot |
P19235-1 (EPOR) | Y489 | Uniprot |
P19235-1 (EPOR) | Y504 | Uniprot |
P27361 (MAPK3) | Y204 | Uniprot |
P28482 (MAPK1) | Y187 | Uniprot |
P40763-2 (STAT3) | Y704 | Uniprot |
P40763-3 (STAT3) | Y705 | Uniprot |
P41597 (CCR2) | Y139 | Uniprot |
P42224 (STAT1) | Y701 | Uniprot |
P42229-1 (STAT5A) | Y694 | Uniprot |
P42680 (TEC) | Y519 | Uniprot |
P46527 (CDKN1B) | Y88 | Uniprot |
P51692 (STAT5B) | Y699 | Uniprot |
P62993 (GRB2) | Y7 | Uniprot |
P62993 (GRB2) | Y37 | Uniprot |
P62993 (GRB2) | Y52 | Uniprot |
P62993 (GRB2) | Y209 | Uniprot |
P78324-1 (SIRPA) | Y496 | Uniprot |
P78347 (GTF2I) | Y248 | Uniprot |
P84243 (H3F3B) | Y42 | Uniprot |
Q13153 (PAK1) | Y153 | Uniprot |
Q13153 (PAK1) | Y201 | Uniprot |
Q13153 (PAK1) | Y285 | Uniprot |
Q14765 (STAT4) | Y693 | Uniprot |
Q15118 (PDK1) | Y243 | Uniprot |
Q15910 (EZH2) | Y641 | Uniprot |
Q92888 (ARHGEF1) | Y738 | Uniprot |
Q99683 (MAP3K5) | Y718 | Uniprot |
Q9UGK3 (STAP2) | Y22 | Uniprot |
Q9UGK3-2 (STAP2) | Y250 | Uniprot |
Q9UGK3 (STAP2) | Y310 | Uniprot |
Q9UGK3 (STAP2) | Y322 | Uniprot |
Q9UQC2 (GAB2) | Y643 | Uniprot |
研究背景
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.
Endomembrane system>Peripheral membrane protein. Cytoplasm. Nucleus.
Ubiquitously expressed throughout most tissues.
Interacts with EPOR, LYN, SIRPA, SH2B1 and TEC (By similarity). Interacts with IL23R. Interacts with SKB1. Interacts with STAM2. Interacts with IFNGR2 (via intracellular domain). Interacts with LEPR (Isoform B) (By similarity). Interacts with HSP90AB1; promotes functional activation in a heat shock-dependent manner. Interacts with STRA6. Interacts with RHEX; this interaction occurs in a erythropoietin (EPO)-dependent manner.
Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1 (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
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