产品: 磷酸化 eEF2K (Ser491/Ser492) 抗体
货号: AF3785
描述: Rabbit polyclonal antibody to Phospho-eEF2K (Ser491/Ser492)
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 82kD(Calculated).
蛋白号: O00418
RRID: AB_2847099

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-eEF2K (Ser491/Ser492) Antibody detects endogenous levels of eEF2K only when phosphorylated at Ser491/Ser492.
RRID:
AB_2847099
引用格式: Affinity Biosciences Cat# AF3785, RRID:AB_2847099.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C86191; Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; EC 2.7.11.20; eEF 2 kinase; eEF 2K; eEF-2 kinase; eEF-2K; Eef2k; EEF2K protein; EF2K_HUMAN; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; fa04b08; HSU93850; kinase eEF2K; MGC45041; SMEF2K; wu:fa04b08; zgc:100973;

抗原和靶标

免疫原:

A synthesized peptide derived from human eEF2K around the phosphorylation site of Ser491/Ser492.

Uniprot:
基因/基因ID:
序列:
MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

翻译修饰 - O00418 作为底物

Site PTM Type Enzyme
A2 Acetylation
S18 Phosphorylation
S27 Phosphorylation
S31 Phosphorylation
Y37 Phosphorylation
K58 Ubiquitination
Y59 Phosphorylation
Y60 Phosphorylation
S61 Phosphorylation O00418 (EEF2K)
K65 Ubiquitination
S66 Phosphorylation O00418 (EEF2K)
Y69 Phosphorylation
S70 Phosphorylation
S71 Phosphorylation
S72 Phosphorylation P42345 (MTOR)
S74 Phosphorylation P42345 (MTOR)
S78 Phosphorylation O00418 (EEF2K) , P54646 (PRKAA2) , Q13131 (PRKAA1) , Q96QT4 (TRPM7)
K82 Ubiquitination
K86 Ubiquitination
K93 Ubiquitination
K132 Ubiquitination
S135 Phosphorylation
K151 Ubiquitination
K162 Ubiquitination
K170 Ubiquitination
R178 Methylation
K202 Ubiquitination
K223 Ubiquitination
K235 Ubiquitination
K238 Ubiquitination
S241 Phosphorylation
S243 Phosphorylation
T340 Phosphorylation
K341 Ubiquitination
S345 Phosphorylation
K347 Ubiquitination
T348 Phosphorylation O00418 (EEF2K)
T353 Phosphorylation O00418 (EEF2K)
S359 Phosphorylation P06493 (CDK1) , O15264 (MAPK13)
T364 Phosphorylation
S366 Phosphorylation Q15418 (RPS6KA1) , Q13131 (PRKAA1) , P17612 (PRKACA) , P54646 (PRKAA2) , O00418 (EEF2K) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2)
S377 Phosphorylation Q16644 (MAPKAPK3) , P49137 (MAPKAPK2) , Q8IW41 (MAPKAPK5)
S380 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation P45984 (MAPK9) , P45983 (MAPK8) , P53778 (MAPK12) , Q15759 (MAPK11) , O15264 (MAPK13) , O00418 (EEF2K) , Q16539 (MAPK14)
S398 Phosphorylation Q13131 (PRKAA1) , P54646 (PRKAA2)
S399 Phosphorylation
T401 Phosphorylation
S416 Phosphorylation
S435 Phosphorylation
S438 Phosphorylation
S441 Phosphorylation O00418 (EEF2K)
Y443 Phosphorylation
S445 Phosphorylation O00418 (EEF2K)
K447 Ubiquitination
S462 Phosphorylation
K467 Sumoylation
Y468 Phosphorylation
S470 Phosphorylation
S474 Phosphorylation O00418 (EEF2K)
S477 Phosphorylation
S478 Phosphorylation
K485 Ubiquitination
S491 Phosphorylation O00418 (EEF2K)
S492 Phosphorylation
S500 Phosphorylation O00418 (EEF2K) , P17612 (PRKACA)
K516 Ubiquitination
K517 Ubiquitination
K520 Ubiquitination
S521 Phosphorylation
K596 Ubiquitination
Y600 Phosphorylation
K603 Ubiquitination
S627 Phosphorylation
K684 Ubiquitination
K705 Ubiquitination
K715 Ubiquitination

翻译修饰 - O00418 作为激酶

Substrate Site Source
O00418 (EEF2K) S61 Uniprot
O00418 (EEF2K) S66 Uniprot
O00418 (EEF2K) S78 Uniprot
O00418 (EEF2K) T348 Uniprot
O00418 (EEF2K) T353 Uniprot
O00418 (EEF2K) S366 Uniprot
O00418 (EEF2K) S396 Uniprot
O00418 (EEF2K) S441 Uniprot
O00418 (EEF2K) S445 Uniprot
O00418 (EEF2K) S474 Uniprot
O00418 (EEF2K) S491 Uniprot
O00418 (EEF2K) S500 Uniprot
P13639 (EEF2) T54 Uniprot
P13639 (EEF2) T57 Uniprot
P13639 (EEF2) T59 Uniprot

研究背景

功能:

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

翻译修饰:

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

亚基结构:

Monomer or homodimer (Probable). Interacts with Calmodulin/CALM1; this interaction is strictly required for phosphorylation activity.

蛋白家族:

Belongs to the protein kinase superfamily. Alpha-type protein kinase family.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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