Phospho-Perilipin-1 (Ser522) Antibody - #AF3834

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产品: 磷酸化 Perilipin-1 (Ser522) 抗体
货号: AF3834
描述: Rabbit polyclonal antibody to Phospho-Perilipin-1 (Ser522)
应用: IHC
文献验证:
反应: Human, Mouse, Rat
分子量: 56kD(Calculated).
蛋白号: O60240
RRID: AB_2847148

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Perilipin-1 (Ser522) Antibody detects endogenous levels of Perilipin-1 only when phosphorylated at Ser522.
RRID:
AB_2847148
引用格式: Affinity Biosciences Cat# AF3834, RRID:AB_2847148.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin A; Perilipin; Perilipin-1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Perilipin-1 around the phosphorylation site of Ser522.

Uniprot:

O60240(PLIN1_HUMAN) >>Visit HPA database.

基因/基因ID:
PLIN1(5346)
表达:
O60240 PLIN1_HUMAN:

Detected in adipocytes from white adipose tissue (at protein level) (PubMed:27832861). Detected in visceral adipose tissue and mammary gland (PubMed:9521880).

序列:
MAVNKGLTLLDGDLPEQENVLQRVLQLPVVSGTCECFQKTYTSTKEAHPLVASVCNAYEKGVQSASSLAAWSMEPVVRRLSTQFTAANELACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESAPAPGHQQAQKSPKAKPSLLSRVGALTNTLSRYTVQTMARALEQGHTVAMWIPGVVPLSSLAQWGASVAMQAVSRRRSEVRVPWLHSLAAAQEEDHEDQTDTEGEDTEEEEELETEENKFSEVAALPGPRGLLGGVAHTLQKTLQTTISAVTWAPAAVLGMAGRVLHLTPAPAVSSTKGRAMSLSDALKGVTDNVVDTVVHYVPLPRLSLMEPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRVSDSFFRPSVMEPILGRTHYSQLRKKS

研究背景

功能:

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.

翻译修饰:

Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity).

细胞定位:

Endoplasmic reticulum. Lipid droplet.
Note: Lipid droplet surface-associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.

蛋白家族:

Belongs to the perilipin family.

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

文献引用

1). Hormone-sensitive lipase protects adipose triglyceride lipase-deficient mice from lethal lipotoxic cardiomyopathy. JOURNAL OF LIPID RESEARCH, 2022 (PubMed: 35283217) [IF=5.0]

Application: WB    Species: Mice    Sample: cardiac tissues

Supplemental Fig. S1. Phosphorylation of PLIN1 in cardiac tissues analyzed using an antibody specific to phosphorylated PLIN1. Twenty μg of the protein sample was subjected to SDS-PAGE, transferred to nitrocellulose membranes, and probed with the antibodies. Fat indicates gonadal adipose tissues used as a positive control. Four mice per group were analyzed and representative images have been presented.
2). A label-free amperometric immunosensor with improved electrocatalytic 3D braided AuPtCu-SWCNTs@MoS2-rGO for human growth differentiation factor-15 detection. Analytical Methods, 2022 (PubMed: 35315459) [IF=2.7]

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