产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3899, RRID:AB_2847213.
展开/折叠
DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5; Smad5; SMAD5_HUMAN;
抗原和靶标
A synthesized peptide derived from human Smad5 around the phosphorylation site of Ser463/465.
- Q99717 SMAD5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSMASLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSSPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFHQPNNTPFPLSPNSPYPPSPASSTYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKSRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNFHHGFHPTTVCKIPSSCSLKIFNNQEFAQLLAQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPLNPISSVS
翻译修饰 - Q99717 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| K117 | Sumoylation | Uniprot | |
| K119 | Sumoylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot |
研究背景
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cytoplasm. Nucleus.
Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Ubiquitous.
May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3. Interacts with WWP1. Interacts with TMEM119 (By similarity). Interacts with ZNF8. Interacts with RANBP3L.
Belongs to the dwarfin/SMAD family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
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