Phospho-UBF1 (Ser484) Antibody - #AF3925

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产品: 磷酸化 UBF1 (Ser484) 抗体
货号: AF3925
描述: Rabbit polyclonal antibody to Phospho-UBF1 (Ser484)
应用: IHC
反应: Human, Mouse, Rat
分子量: 89kD(Calculated).
蛋白号: P17480
RRID: AB_2847648

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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WB Handbook
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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-UBF1 (Ser484) Antibody detects endogenous levels of UBF1 only when phosphorylated at Ser484.
RRID:
AB_2847648
引用格式: Affinity Biosciences Cat# AF3925, RRID:AB_2847648.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human UBF1 around the phosphorylation site of Ser484.

Uniprot:

P17480(UBF1_HUMAN) >>Visit HPA database.

基因/基因ID:
UBTF(7343)
序列:
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN

翻译修饰 - P17480 作为底物

Site PTM Type Enzyme
Phosphorylation
M1 Acetylation
T9 Phosphorylation
K17 Ubiquitination
S23 Phosphorylation
K35 Ubiquitination
S44 Phosphorylation
K45 Ubiquitination
K47 Ubiquitination
K57 Ubiquitination
K61 Acetylation
K61 Ubiquitination
K69 Ubiquitination
K71 Ubiquitination
K97 Ubiquitination
K107 Ubiquitination
K114 Acetylation
K114 Sumoylation
K114 Ubiquitination
T117 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
K126 Ubiquitination
K132 Ubiquitination
K144 Ubiquitination
S147 Phosphorylation
Y150 Phosphorylation
K151 Ubiquitination
K160 Sumoylation
K160 Ubiquitination
K169 Ubiquitination
K191 Ubiquitination
K198 Ubiquitination
K200 Ubiquitination
T201 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
Y207 Phosphorylation
T208 Phosphorylation
K211 Ubiquitination
K216 Ubiquitination
K224 Ubiquitination
K232 Methylation
K232 Ubiquitination
S238 Phosphorylation
K240 Ubiquitination
K244 Ubiquitination
K248 Ubiquitination
K254 Methylation
K254 Ubiquitination
K266 Ubiquitination
S273 Phosphorylation
K279 Ubiquitination
K284 Acetylation
K284 Sumoylation
K284 Ubiquitination
S306 Phosphorylation
Y308 Phosphorylation
S329 Phosphorylation
K333 Ubiquitination
S336 Phosphorylation
K338 Ubiquitination
K352 Acetylation
K352 Ubiquitination
S364 Phosphorylation
K378 Ubiquitination
K384 Ubiquitination
K385 Acetylation
T388 Phosphorylation
S389 Phosphorylation P24941 (CDK2)
S392 Phosphorylation
K394 Acetylation
K401 Acetylation
K406 Acetylation
S412 Phosphorylation
K421 Ubiquitination
R429 Methylation
S433 Phosphorylation
S435 Phosphorylation
R443 Methylation
S449 Phosphorylation
Y456 Phosphorylation
K464 Sumoylation
K480 Ubiquitination
S484 Phosphorylation P24941 (CDK2) , P11802 (CDK4)
S495 Phosphorylation
Y500 Phosphorylation
S546 Phosphorylation
K559 Acetylation
S584 Phosphorylation
K594 Ubiquitination
S602 Phosphorylation
S610 Phosphorylation
K624 Ubiquitination
S638 Phosphorylation
R642 Methylation
K646 Ubiquitination
Y648 Phosphorylation
S655 Phosphorylation
S666 Phosphorylation

研究背景

功能:

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

翻译修饰:

Phosphorylated and activated by PIK3CA.

细胞定位:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.

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