| 产品: | 磷酸化 EPB41 (Tyr660/Tyr418) 抗体 |
| 货号: | AF3943 |
| 描述: | Rabbit polyclonal antibody to Phospho-EPB41 (Tyr660/Tyr418) |
| 应用: | ELISA(peptide) |
| 反应: | Human, Mouse |
| 分子量: | 97kD(Calculated). |
| 蛋白号: | P11171 |
| RRID: | AB_2847666 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3943, RRID:AB_2847666.
展开/折叠
4.1R; 41_HUMAN; Band 4.1; E41P; EL 1; EL1; EL1 gene; Elliptocytosis 1; Elliptocytosis 1 RH linked; EPB 4.1; EPB 41; EPB4.1; Epb41; Erythrocyte membrane protein band 4.1 (elliptocytosis 1 RH linked); Erythrocyte membrane protein band 4.1; Erythrocyte surface protein band 4.1; HE; P4.1; Protein 4.1; Protein 4.1, red blood cell type;
抗原和靶标
A synthesized peptide derived from human EPB41 around the phosphorylation site of Tyr660/418.
- P11171 41_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTIQSELGDYDPELHGVDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGEQEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLTSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAVDSADRSPRPTSAPAITQGQVAEGGVLDASAKKTVVPKAQKETVKAEVKKEDEPPEQAEPEPTEAWKVEKTHIEVTVPTSNGDQTQKLAEKTEDLIRMRKKKRERLDGENIYIRHSNLMLEDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTLNINGQIPTGEGPPLVKTQTVTISDNANAVKSEIPTKDVPIVHTETKTITYEAAQTDDNSGDLDPGVLLTAQTITSETPSSTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQVLVQAIKEAKEQHPDMSVTKVVVHQETEIADE
翻译修饰 - P11171 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| R77 | Methylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| K361 | Methylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K366 | Acetylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K372 | Methylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| T378 | Phosphorylation | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| K466 | Acetylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| S491 | O-Glycosylation | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| R526 | Methylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| T559 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| S578 | Phosphorylation | Uniprot | |
| Y660 | Phosphorylation | P00533 (EGFR) | Uniprot |
| S664 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| T710 | Phosphorylation | Uniprot | |
| S712 | Phosphorylation | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| T736 | Phosphorylation | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| Y767 | Phosphorylation | Uniprot | |
| T786 | Phosphorylation | Uniprot | |
| T794 | Phosphorylation | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T823 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| T851 | Phosphorylation | Uniprot | |
| T859 | Phosphorylation | Uniprot |
研究背景
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.
O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.
Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Nucleus.
Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Interacts with calmodulin. Interacts with CENPJ. Interacts with DLG1. Also found to associate with contractile apparatus and tight junctions. Interacts with NUMA1; this interaction is negatively regulated by CDK1 during metaphase and promotes anaphase-specific localization of NUMA1 in symmetrically dividing cells. Interacts with ATP2B1; regulates small intestinal calcium absorption through regulation of membrane expression of ATP2B1 (By similarity).
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