Lamin B1 Mouse Monoclonal Antibody - #BF8009

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产品: Lamin B1 小鼠 单克隆 抗体
货号: BF8009
描述: Mouse monoclonal antibody to Lamin B1
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
分子量: 66kD; 66kD(Calculated).
蛋白号: P20700

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   规格 价格 库存
 50ul RMB¥ 900 现货
 100ul RMB¥ 1500 现货
 200ul RMB¥ 2500 现货

货期: 当天发货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Monoclonal [AFfirm8009]
特异性:
Lamin B1 Mouse Monoclonal Antibody detects endogenous levels of total Lamin B1.
偶联:
Unconjugated. 130
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;

抗原和靶标

免疫原:

A synthesized peptide derived from human Lamin B1.

Uniprot:

P20700(LMNB1_HUMAN) >>Visit HPA database.

基因/基因ID:
LMNB1(4001)
序列:
MATATPVPPRMGSRAGGPTTPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERAKLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSLTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM

研究背景

功能:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

翻译修饰:

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

细胞定位:

Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

文献引用

1). ANXA1 promotes tumor immune evasion by binding PARP1 and upregulating Stat3-induced expression of PD-L1 in multiple cancers. Cancer Immunology Research, 2023 (PubMed: 37566399) [IF=8.1]
2). Honokiol suppresses the aberrant interactions between renal resident macrophages and tubular epithelial cells in lupus nephritis through the NLRP3/IL-33/ST2 axis. Cell Death & Disease, 2023 (PubMed: 36859530) [IF=8.1]

Application: WB    Species: Mouse    Sample:

Fig. 5: Effects of Honokiol (HNK) on the renal levels of the NLRP3 inflammasome and the ST2 and NF-κB proteins in MpJ/lpr mice.
3). WLJP-025p, a homogeneous Lonicera japonica polysaccharide, attenuates atopic dermatitis by regulating the MAPK/NFκB/AP-1 axis via Act1. International journal of biological macromolecules, 2024 (PubMed: 38016605) [IF=7.7]
4). Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis. Journal of orthopaedic translation, 2024 (PubMed: 39007035) [IF=5.9]
5). Panax notoginseng Saponins Activate Nuclear Factor Erythroid 2-Related Factor 2 to Inhibit Ferroptosis and Attenuate Inflammatory Injury in Cerebral Ischemia-Reperfusion. The American journal of Chinese medicine, 2024 (PubMed: 38699996) [IF=4.8]
6). Improving hyperglycemic effect of FGF-21 is associated with alleviating inflammatory state in diabetes. International Immunopharmacology, 2018 (PubMed: 29414665) [IF=4.8]
7). FGF-21 Elevated IL-10 Production to Correct LPS-Induced Inflammation. INFLAMMATION, 2018 (PubMed: 29427162) [IF=4.5]
8). Astragaloside IV mitigates cerebral ischaemia-reperfusion injury via inhibition of P62/Keap1/Nrf2 pathway-mediated ferroptosis. European Journal of Pharmacology, 2023 (PubMed: 36758783) [IF=4.2]
9). Echinatin mitigates sevoflurane-induced hippocampal neurotoxicity and cognitive deficits through mitigation of iron overload and oxidative stress. PHARMACEUTICAL BIOLOGY, 2022 (PubMed: 36205592) [IF=3.9]

Application: WB    Species: Rat    Sample:

Figure 3. Ech activates Nrf2 in hippocampal neurons and hippocampus of aged rats. (A and B) The mRNA and protein levels of Nrf2 in vitro were detected by qRT-PCR and western blot. (C and D) The mRNA and protein levels of Nrf2 in vivo were detected by qRT-PCR and western blot. Compared with the Sev group
10). Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FOOD AND CHEMICAL TOXICOLOGY, 2017 (PubMed: 28137608) [IF=3.9]

Application: WB    Species: rat    Sample: PC12 cells

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