产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;
抗原和靶标
A synthesized peptide derived from human Lamin B1.
- P20700 LMNB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATATPVPPRMGSRAGGPTTPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERAKLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSLTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM
翻译修饰 - P20700 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
T3 | Phosphorylation | Uniprot | |
T5 | Phosphorylation | Uniprot | |
R10 | Methylation | Uniprot | |
S13 | Phosphorylation | Uniprot | |
R14 | Methylation | Uniprot | |
T19 | Phosphorylation | Uniprot | |
T20 | Phosphorylation | Uniprot | |
S23 | Phosphorylation | P06493 (CDK1) | Uniprot |
T25 | Phosphorylation | Uniprot | |
S28 | Phosphorylation | Uniprot | |
K33 | Acetylation | Uniprot | |
K33 | Ubiquitination | Uniprot | |
R42 | Methylation | Uniprot | |
Y46 | Phosphorylation | Uniprot | |
K49 | Acetylation | Uniprot | |
K49 | Ubiquitination | Uniprot | |
S58 | Phosphorylation | Uniprot | |
K79 | Acetylation | Uniprot | |
K79 | Ubiquitination | Uniprot | |
Y82 | Phosphorylation | Uniprot | |
T84 | Phosphorylation | Uniprot | |
K102 | Acetylation | Uniprot | |
K102 | Sumoylation | Uniprot | |
K102 | Ubiquitination | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K111 | Acetylation | Uniprot | |
K111 | Ubiquitination | Uniprot | |
K123 | Acetylation | Uniprot | |
K123 | Ubiquitination | Uniprot | |
K124 | Ubiquitination | Uniprot | |
S126 | Phosphorylation | Uniprot | |
K134 | Acetylation | Uniprot | |
K134 | Ubiquitination | Uniprot | |
Y138 | Phosphorylation | Uniprot | |
S144 | Phosphorylation | Uniprot | |
K145 | Ubiquitination | Uniprot | |
K156 | Acetylation | Uniprot | |
K156 | Ubiquitination | Uniprot | |
K157 | Acetylation | Uniprot | |
K157 | Ubiquitination | Uniprot | |
S158 | Phosphorylation | Uniprot | |
K167 | Ubiquitination | Uniprot | |
K181 | Acetylation | Uniprot | |
K181 | Ubiquitination | Uniprot | |
K182 | Ubiquitination | Uniprot | |
R197 | Methylation | Uniprot | |
C198 | S-Nitrosylation | Uniprot | |
S200 | Phosphorylation | Uniprot | |
K209 | Acetylation | Uniprot | |
K209 | Ubiquitination | Uniprot | |
S210 | Phosphorylation | Uniprot | |
Y212 | Phosphorylation | Uniprot | |
K222 | Ubiquitination | Uniprot | |
S232 | Phosphorylation | Uniprot | |
Y238 | Phosphorylation | Uniprot | |
K241 | Sumoylation | Uniprot | |
K241 | Ubiquitination | Uniprot | |
R250 | Methylation | Uniprot | |
K261 | Sumoylation | Uniprot | |
K261 | Ubiquitination | Uniprot | |
T267 | Phosphorylation | Uniprot | |
Y268 | Phosphorylation | Uniprot | |
K271 | Acetylation | Uniprot | |
K271 | Ubiquitination | Uniprot | |
S278 | Phosphorylation | Uniprot | |
S279 | Phosphorylation | Uniprot | |
T283 | Phosphorylation | Uniprot | |
S284 | Phosphorylation | Uniprot | |
T285 | Phosphorylation | Uniprot | |
S288 | Phosphorylation | Uniprot | |
R297 | Methylation | Uniprot | |
R299 | Methylation | Uniprot | |
S302 | Phosphorylation | Uniprot | |
S304 | Phosphorylation | Uniprot | |
S305 | Phosphorylation | Uniprot | |
K312 | Acetylation | Uniprot | |
K312 | Ubiquitination | Uniprot | |
K330 | Ubiquitination | Uniprot | |
T340 | Phosphorylation | Uniprot | |
K342 | Ubiquitination | Uniprot | |
Y360 | Phosphorylation | Uniprot | |
S375 | Phosphorylation | Uniprot | |
Y377 | Phosphorylation | Uniprot | |
K379 | Acetylation | Uniprot | |
K379 | Sumoylation | Uniprot | |
K379 | Ubiquitination | Uniprot | |
R387 | Methylation | Uniprot | |
K389 | Sumoylation | Uniprot | |
K389 | Ubiquitination | Uniprot | |
S391 | Phosphorylation | Uniprot | |
S393 | Phosphorylation | P06493 (CDK1) | Uniprot |
S395 | Phosphorylation | P05771 (PRKCB) | Uniprot |
S396 | Phosphorylation | Uniprot | |
T399 | Phosphorylation | Uniprot | |
S401 | Phosphorylation | Uniprot | |
S404 | Phosphorylation | Uniprot | |
S405 | Phosphorylation | P05771 (PRKCB) | Uniprot |
S406 | Phosphorylation | Uniprot | |
S408 | Phosphorylation | Uniprot | |
T411 | Phosphorylation | Uniprot | |
T412 | Phosphorylation | Uniprot | |
K457 | Ubiquitination | Uniprot | |
S460 | Phosphorylation | Uniprot | |
K474 | Ubiquitination | Uniprot | |
S479 | Phosphorylation | Uniprot | |
S481 | Phosphorylation | Uniprot | |
K483 | Acetylation | Uniprot | |
K483 | Ubiquitination | Uniprot | |
K528 | Ubiquitination | Uniprot | |
K532 | Acetylation | Uniprot | |
K532 | Ubiquitination | Uniprot | |
S534 | Phosphorylation | Uniprot | |
K547 | Ubiquitination | Uniprot | |
T575 | Phosphorylation | Uniprot | |
S579 | Phosphorylation | Uniprot |
研究背景
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.
Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.
Belongs to the intermediate filament family.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
文献引用
Application: WB Species: Mouse Sample:
Application: WB Species: mouse Sample: LoVo‑P and HT29‑NC cells
Application: WB Species: Rat Sample:
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