AFfirm™ GFAP Mouse monoclonal Antibody - #BF8023

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产品: GFAP 小鼠 单克隆 抗体
货号: BF8023
描述: Mouse monoclonal antibody to GFAP
应用: WB
反应: Mouse, Rat
分子量: 50kDa; 50kD(Calculated).
蛋白号: P14136

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 100ul RMB¥ 688 2500 现货

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MSDS
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Mouse,Rat
克隆:
Monoclonal [AFfirm8023]
特异性:
GFAP antibody detects endogenous levels of total GFAP.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

wu:fb34h11; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; GFAP; GFAP_HUMAN; gfapl; Glial fibrillary acidic protein; Intermediate filament protein; wu:fk42c12; xx:af506734; zgc:110485;

抗原和靶标

免疫原:

Purified recombinant fragment of human GFAP expressed in E. Coli.

Uniprot:

P14136(GFAP_HUMAN) >>Visit HPA database.

基因/基因ID:
GFAP(2670)
表达:
P14136 GFAP_HUMAN:

Expressed in cells lacking fibronectin.

描述:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
序列:
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

翻译修饰 - P14136 作为底物

Site PTM Type Enzyme
T7 Phosphorylation Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1)
S8 Phosphorylation P17612 (PRKACA)
R11 Methylation
R12 Methylation
S13 Phosphorylation Q13464 (ROCK1) , P17612 (PRKACA) , Q96GD4 (AURKB)
Y14 Phosphorylation
S17 Phosphorylation
S38 Phosphorylation Q96GD4 (AURKB) , Q13464 (ROCK1) , P17612 (PRKACA)
K95 Acetylation
Y116 Phosphorylation
T131 Phosphorylation
T150 Phosphorylation
K154 Acetylation
Y172 Phosphorylation
K189 Acetylation
K228 Acetylation
T240 Phosphorylation
Y242 Phosphorylation
K260 Acetylation
S305 Phosphorylation
Y324 Phosphorylation
K339 Acetylation
Y349 Phosphorylation

研究背景

功能:

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

翻译修饰:

Phosphorylated by PKN1.

细胞定位:

Cytoplasm.
Note: Associated with intermediate filaments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in cells lacking fibronectin.

亚基结构:

Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

文献引用

1). Total flavones of Rhododendron induce the transformation of A1/A2 astrocytes via promoting the release of CBS-produced H2S. PHYTOMEDICINE, 2023 (PubMed: 36701996) [IF=7.9]
2). Aucubin improves chronic unpredictable mild stress-induced depressive behavior in mice via the GR/NF-κB/NLRP3 axis. International Immunopharmacology, 2023 (PubMed: 37523973) [IF=5.6]
3). Inhibition of HDAC6 alleviates cancer‑induced bone pain by reducing the activation of NLRP3 inflammasome. International journal of molecular medicine, 2024 (PubMed: 37997785) [IF=5.4]

Application: IF/ICC    Species: Rat    Sample: spinal cord

Figure 2 The effect of TSA on the spinal HDAC6 activation and behaviors tests. Representative immunofluorescence staining images (A) and quantitative analysis (B) for HDAC6 and GFAP in the spinal cord. Scale bar, 20 μm. Representative western blot bands (C) and quantitative analysis (D) of pHDAC6 (Ser22) protein in spinal cord. Data were presented as mean ± SD (n=3). *P

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