AFfirm™ ACTR3 Mouse Monoclonal Antibody - #BF8074

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产品: ACTR3 小鼠 单克隆 抗体
货号: BF8074
描述: Mouse monoclonal antibody to ACTR3
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 47kDa; 47kD(Calculated).
蛋白号: P61158

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   规格 价格 库存
 100ul RMB¥ 688 2300 现货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Mouse
应用:
WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Monoclonal [AFfirm8074]
特异性:
ACTR3 Antibody detects endogenous levels of total ACTR3.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Actin like protein 3; Actin related protein 3; ACTR3; ARP3 actin related protein 3 homolog (yeast);

抗原和靶标

免疫原:
Uniprot:

P61158(ARP3_HUMAN) >>Visit HPA database.

基因/基因ID:
ACTR3(10096)
描述:
Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).
序列:
MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

翻译修饰 - P61158 作为底物

Site PTM Type Enzyme
A2 Acetylation
C8 S-Nitrosylation
C12 S-Nitrosylation
Y16 Phosphorylation
K18 Ubiquitination
K38 Ubiquitination
K42 Ubiquitination
K53 Acetylation
K53 Ubiquitination
K69 Ubiquitination
K75 Ubiquitination
K99 Ubiquitination
Y100 Phosphorylation
Y109 Phosphorylation
T113 Phosphorylation
T119 Phosphorylation
S133 Phosphorylation
Y140 Phosphorylation
S152 Phosphorylation
Y184 Phosphorylation
K191 Ubiquitination
T201 Phosphorylation
Y202 Phosphorylation
K225 Ubiquitination
K228 Ubiquitination
Y231 Phosphorylation
S232 Phosphorylation
Y233 Phosphorylation
K240 Acetylation
K240 Ubiquitination
K244 Acetylation
K244 Ubiquitination
K251 Acetylation
K251 Ubiquitination
K254 Acetylation
K254 Ubiquitination
K264 Sumoylation
K264 Ubiquitination
K265 Ubiquitination
S268 Phosphorylation
K317 Ubiquitination
S322 Phosphorylation
S325 Phosphorylation
T326 Phosphorylation
K348 Ubiquitination
S350 Phosphorylation
S354 Phosphorylation
K359 Ubiquitination
K361 Ubiquitination
K398 Ubiquitination
Y400 Phosphorylation
S418 Phosphorylation

研究背景

功能:

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.

细胞定位:

Cytoplasm>Cytoskeleton. Cell projection. Nucleus.
Note: In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Interacts weakly with MEFV.

蛋白家族:

Belongs to the actin family. ARP3 subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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