产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
AIFM1; AIFM1_HUMAN; Apoptosis inducing factor 1, mitochondrial; Apoptosis inducing factor; Apoptosis inducing factor, mitochondrion associated, 1; Apoptosis-inducing factor 1; CMTX4; COWCK; COXPD6; Harlequin; Hq; mAIF; MGC111425; MGC5706; mitochondrial; Neuropathy, axonal motor-sensory, with deafness and mental retardation; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome); PDCD 8; PDCD8; Programmed cell death 8 (apoptosis inducing factor); Programmed cell death 8; Programmed cell death 8 isoform 1; Programmed cell death 8 isoform 2; Programmed cell death 8 isoform 3; Programmed cell death protein 8; Programmed cell death protein 8 mitochondrial; Programmed cell death protein 8 mitochondrial precursor; Programmed cell death protein 8 mitochondrial precursor; Striatal apoptosis inducing factor;
抗原和靶标
A synthesized peptide derived from human AIFM1, corresponding to a region within C-terminal amino acids.
Expressed in all tested tissues (PubMed:16644725). Detected in muscle and skin fibroblasts (at protein level) (PubMed:23217327).
Brain specific.
Expressed in all tested tissues except brain.
Isoform 5 is frequently down-regulated in human cancers.
- O95831 AIFM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRCGGLAAGALKQKLVPLVRTVCVRSPRQRNRLPGNLFQRWHVPLELQMTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED
翻译修饰 - O95831 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S56 | Phosphorylation | Uniprot | |
S57 | Phosphorylation | Uniprot | |
S60 | Phosphorylation | Uniprot | |
T87 | Phosphorylation | Uniprot | |
Y95 | Phosphorylation | Uniprot | |
S100 | Phosphorylation | Uniprot | |
T105 | Phosphorylation | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K111 | Ubiquitination | Uniprot | |
S116 | Phosphorylation | Uniprot | |
S118 | Phosphorylation | Uniprot | |
S176 | Phosphorylation | Uniprot | |
S182 | Phosphorylation | Uniprot | |
T188 | Phosphorylation | Uniprot | |
K189 | Ubiquitination | Uniprot | |
T190 | Phosphorylation | Uniprot | |
K232 | Ubiquitination | Uniprot | |
K244 | Acetylation | Uniprot | |
K255 | Ubiquitination | Uniprot | |
T263 | Phosphorylation | Uniprot | |
S266 | Phosphorylation | Uniprot | |
S268 | Phosphorylation | Uniprot | |
K278 | Ubiquitination | Uniprot | |
S279 | Phosphorylation | Uniprot | |
S292 | Phosphorylation | Uniprot | |
K295 | Acetylation | Uniprot | |
T328 | Phosphorylation | Uniprot | |
K337 | Acetylation | Uniprot | |
Y347 | Phosphorylation | Uniprot | |
S371 | Phosphorylation | Uniprot | |
S375 | Phosphorylation | Uniprot | |
S376 | Phosphorylation | Uniprot | |
S416 | Phosphorylation | Uniprot | |
Y443 | Phosphorylation | Uniprot | |
K518 | Ubiquitination | Uniprot | |
S519 | Phosphorylation | Uniprot | |
T521 | Phosphorylation | Uniprot | |
S524 | Phosphorylation | Uniprot | |
T526 | Phosphorylation | Uniprot | |
S530 | Phosphorylation | Uniprot | |
S532 | Phosphorylation | Uniprot | |
T542 | Phosphorylation | Uniprot | |
S546 | Phosphorylation | Uniprot | |
T547 | Phosphorylation | Uniprot | |
R569 | Methylation | Uniprot | |
K593 | Acetylation | Uniprot | |
K593 | Ubiquitination | Uniprot | |
K606 | Ubiquitination | Uniprot |
研究背景
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (By similarity). Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import.
Has NADH oxidoreductase activity. Does not induce nuclear apoptosis.
Pro-apoptotic isoform.
Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.
Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm>Perinuclear region.
Note: Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:15775970). Colocalizes with EIF3G in the nucleus and perinuclear region (PubMed:17094969).
Mitochondrion intermembrane space. Mitochondrion inner membrane.
Note: Has a stronger membrane anchorage than isoform 1.
Mitochondrion. Cytoplasm>Cytosol.
Note: In pro-apoptotic conditions, is released from mitochondria to cytosol in a calpain/cathepsin-dependent manner.
Cytoplasm.
Expressed in all tested tissues. Detected in muscle and skin fibroblasts (at protein level).
Brain specific.
Expressed in all tested tissues except brain.
Isoform 5 is frequently down-regulated in human cancers.
Monomer (oxidized form). Homodimer (reduced form). Upon reduction with NADH, undergoes dimerization and forms tight, long-lived FADH2-NAD charge transfer complexes (CTC) resistant to oxidation. Also dimerizes with isoform 3 preventing its release from mitochondria. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Interacts with PRELID1. Interacts with CHCHD4; the interaction increases in presence of NADH.
Belongs to the FAD-dependent oxidoreductase family.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
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