产品: Prohibitin 小鼠 单克隆 抗体
货号: BF8085
描述: Mouse monoclonal antibody to Prohibitin
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 30kDa; 30kD(Calculated).
蛋白号: P35232

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 100ul RMB¥ 688 2300 现货

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产品描述

来源:
Mouse
应用:
WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Monoclonal [AFfirm8085]
特异性:
Prohibitin Antibody detects endogenous levels of total Prohibitin.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB_HUMAN; PHB1; Prohibitin;

抗原和靶标

免疫原:

A synthesized peptide derived from human Prohibitin, corresponding to a region within the internal amino acids.

Uniprot:
基因/基因ID:
表达:
P35232 PHB_HUMAN:

Widely expressed in different tissues.

描述:
The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).
序列:
MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ

翻译修饰 - P35232 作为底物

Site PTM Type Enzyme
A2 Acetylation
K4 Acetylation
K4 Methylation
K4 Ubiquitination
K11 Methylation
R41 Methylation
S82 Phosphorylation
K83 Ubiquitination
T91 Phosphorylation
R93 Methylation
S101 Phosphorylation
T108 Phosphorylation
S109 Phosphorylation
Y114 Phosphorylation P06213 (INSR)
S121 O-Glycosylation
T123 Phosphorylation
K128 Acetylation
K128 Ubiquitination
S151 Phosphorylation
K177 Ubiquitination
K186 Ubiquitination
K202 Acetylation
K202 Ubiquitination
K207 Acetylation
K208 Ubiquitination
S213 Phosphorylation
S218 Phosphorylation
K219 Ubiquitination
K240 Ubiquitination
Y249 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
T258 O-Glycosylation
T258 Phosphorylation P31749 (AKT1)
Y259 Phosphorylation
S265 Phosphorylation

研究背景

功能:

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

细胞定位:

Mitochondrion inner membrane. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed in different tissues.

亚基结构:

Interacts with PHB2. Interacts with STOML2.

蛋白家族:

Belongs to the prohibitin family.

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