产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
cell proliferation-inducing protein 59; GLNA; GLNA_HUMAN; GLNS; GLUL; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamate--ammonia ligase; glutamine synthase; Glutamine synthetase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43;
抗原和靶标
- P15104 GLNA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
翻译修饰 - P15104 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| C183 | S-Nitrosylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| C359 | S-Nitrosylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| K372 | Acetylation | Uniprot | |
| K372 | Ubiquitination | Uniprot |
研究背景
Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis.
Palmitoylated; undergoes autopalmitoylation.
Ubiquitinated by ZNRF1.
Cytoplasm>Cytosol. Microsome. Mitochondrion. Cell membrane>Lipid-anchor.
Note: Mainly localizes in the cytosol, with a fraction associated with the cell membrane.
Expressed in endothelial cells.
Decamer; composed of two pentamers. Interacts with PALMD (By similarity). Interacts with RHOJ.
Belongs to the glutamine synthetase family.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Metabolism > Amino acid metabolism > Arginine biosynthesis.
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Energy metabolism > Nitrogen metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.