产品: AMPK beta 1 小鼠 单克隆 抗体
货号: BF8178
描述: Mouse monoclonal antibody to AMPK beta 1
应用: WB IHC
反应: Human
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 38kDa; 30kD(Calculated).
蛋白号: Q9Y478

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm8178]
特异性:
AMPK beta 1 Antibody detects endogenous levels of total AMPK beta 1.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1300015D22Rik; 5''-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase beta-1 subunit; AAKB1_HUMAN; AMP-activated protein kinase beta subunit; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; AU021155; E430008F22; HAMPKb; MGC17785; PRKAB1; Protein kinase AMP activated non catalytic subunit beta 1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA.
序列:
MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

翻译修饰 - Q9Y478 作为底物

Site PTM Type Enzyme
G2 Myristoylation
T4 Phosphorylation
S5 Phosphorylation
S6 Phosphorylation
T19 Phosphorylation
S24 Phosphorylation Q13131 (PRKAA1)
S25 Phosphorylation
T28 Phosphorylation
K35 Ubiquitination
S40 Phosphorylation
S49 Phosphorylation
K53 Ubiquitination
K57 Ubiquitination
K72 Ubiquitination
T80 Phosphorylation Q13131 (PRKAA1)
K89 Ubiquitination
Y92 Phosphorylation
S94 Phosphorylation
S96 Phosphorylation
S101 Phosphorylation
K102 Ubiquitination
S108 Phosphorylation Q13131 (PRKAA1)
S138 Phosphorylation
S144 Phosphorylation
T148 Phosphorylation Q13131 (PRKAA1)
K157 Ubiquitination
T158 Phosphorylation Q13131 (PRKAA1)
S170 Phosphorylation
S174 Phosphorylation Q13131 (PRKAA1)
S177 Phosphorylation Q13131 (PRKAA1)
S180 Phosphorylation
S181 Phosphorylation
S182 Phosphorylation
Y187 Phosphorylation
K195 Ubiquitination
S252 Phosphorylation
T254 Phosphorylation
Y261 Phosphorylation
K268 Ubiquitination

翻译修饰 - Q9Y478 作为激酶

Substrate Site Source
P14859 (POU2F1) S335 Uniprot
P29474 (NOS3) S1177 Uniprot

研究背景

功能:

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

翻译修饰:

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

亚基结构:

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

蛋白家族:

The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.

Belongs to the 5'-AMP-activated protein kinase beta subunit family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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