产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
1300015D22Rik; 5''-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase beta-1 subunit; AAKB1_HUMAN; AMP-activated protein kinase beta subunit; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; AU021155; E430008F22; HAMPKb; MGC17785; PRKAB1; Protein kinase AMP activated non catalytic subunit beta 1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1;
抗原和靶标
- Q9Y478 AAKB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI
翻译修饰 - Q9Y478 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S25 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| T80 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K89 | Ubiquitination | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S138 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K157 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S170 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S177 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S180 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y187 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| K268 | Ubiquitination | Uniprot |
研究背景
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.
The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
Belongs to the 5'-AMP-activated protein kinase beta subunit family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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