AFfirm™ GPR120 Mouse Monoclonal Antibody - #BF8192

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产品: GPR120 小鼠 单克隆 抗体
货号: BF8192
描述: Mouse monoclonal antibody to GPR120
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 42 kDa; 42kD(Calculated).
蛋白号: Q5NUL3

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Monoclonal [AFfirm8192]
特异性:
GPR120 Antibody detects endogenous levels of total GPR120.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

G protein coupled receptor 120; G protein coupled receptor 129; G protein coupled receptor GT01; G protein coupled receptor PGR 4; G protein coupled receptor PGR4; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; GPR 120; GPR 129; GPR120; GPR129; GT01; HGNC:19345; MGC119984; O3FA1_HUMAN; O3FAR1; Omega-3 fatty acid receptor 1; PGR 4; PGR4;

抗原和靶标

免疫原:

A synthesized peptide derived from human GPR120, corresponding to a region within the internal amino acids.

Uniprot:

Q5NUL3(FFAR4_HUMAN) >>Visit HPA database.

基因/基因ID:
FFAR4(338557)
表达:
Q5NUL3 FFAR4_HUMAN:

Abundant expression in the intestinal tract. Highly expressed in adipose tissue, small intestine and pancreas.

描述:
GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source
序列:
MSPECARAAGDAPLRSLEQANRTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVCALVLVARRRRRGATACLVLNLFCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTLSGSVTILTLAAVSLERMVCIVHLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVVPQRLPGADQEISICTLIWPTIPGEISWDVSFVTLNFLVPGLVIVISYSKILQTSEHLLDARAVVTHSEITKASRKRLTVSLAYSESHQIRVSQQDFRLFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWPSLFFWVVAFTFANSALNPILYNMTLCRNEWKKIFCCFWFPEKGAILTDTSVKRNDLSIISG

研究背景

功能:

Receptor for medium and long-chain free fatty acids (FFAs). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects, particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1-dependent effect, but independent of the G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Mediates FFA-induced inhibition of apoptosis in enteroendocrine cells. May play a role in the regulation of adipocyte development and differentiation.

翻译修饰:

Phosphorylated. FFA stimulation facilitates phosphorylation.

细胞定位:

Cell membrane>Multi-pass membrane protein.
Note: Colocalized with ARRB2 following DHA treatment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Abundant expression in the intestinal tract. Highly expressed in adipose tissue, small intestine and pancreas.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

文献引用

1). Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway. Acta pharmaceutica Sinica. B, 2025 (PubMed: 40041898) [IF=14.7]

Application: WB    Species: Mouse    Sample:

Figure 5. CpdA increased nuclear translocation of AhR. Immunoprecipitation was performed with whole cell proteins (A, B), cytoplastic proteins (C) or nuclear proteins (D) extracted from Jurkat cells. Anti-β-Arrestin2 (Arrb2), anti-AhR and anti-ARNT were used for pull-down assay. Western blot was used to detect proteins in precipitate. (E) Jurkat cells were treated by CpdA (10 μmol/L) or/and FICZ (0.1 μmol/L) for 24 h. Western blot was used to detect AhR in cytoplastic and nuclear proteins. (F) Proposed actions of CpdA in T-cell. (G, H) T-cells were transfected with siRNAs and stimulated by CD3/CD28 Dynabeads. (G) Cells were detected by immunofluorescence and analyzed as described in Fig. 3F. (H) Western blot was used to detect AhR in cytoplastic and nuclear proteins. Figures show representative result from three (A–C, G) or two (D, E, H) independent experiments. Data are mean ± SD, compared using Student's t test.

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