Phospho-ROCK2 (Tyr722) Antibody - #AF3028

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产品: 磷酸化 ROCK2 (Tyr722) 抗体
货号: AF3028
描述: Rabbit polyclonal antibody to Phospho-ROCK2 (Tyr722)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 165kDa; 161kD(Calculated).
蛋白号: O75116
RRID: AB_2835336

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
Phospho-ROCK2 (Tyr722) Antibody detects endogenous levels of ROCK2 only when phosphorylated at Tyr722.
RRID:
AB_2835336
引用格式: Affinity Biosciences Cat# AF3028, RRID:AB_2835336.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

coiled-coil-containing protein kinase 2; KIAA0619; p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho associated, coiled coil containing protein kinase II; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; ROCK 2; Rock II; Rock2; ROCK2_HUMAN; Rock2m; ROK alpha; ROKalpha;

抗原和靶标

免疫原:
Uniprot:

O75116(ROCK2_HUMAN) >>Visit HPA database.

基因/基因ID:
ROCK2(9475)
表达:
O75116 ROCK2_HUMAN:

Expressed in the brain (at protein level).

描述:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]
序列:
MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75116 作为底物

Site PTM Type Enzyme
T16 Phosphorylation
S25 Phosphorylation
K29 Ubiquitination
Y92 Phosphorylation
K96 Ubiquitination
K121 Ubiquitination
K125 Ubiquitination
K216 Ubiquitination
T249 Phosphorylation
S298 Phosphorylation
K323 Ubiquitination
K355 Ubiquitination
K391 Ubiquitination
T396 Phosphorylation
T414 Phosphorylation O75116 (ROCK2)
S423 Phosphorylation
S425 Phosphorylation
K448 Ubiquitination
K482 Ubiquitination
K492 Ubiquitination
K505 Ubiquitination
K511 Ubiquitination
K537 Ubiquitination
K556 Ubiquitination
T567 Phosphorylation
T573 Phosphorylation
S575 Phosphorylation
T577 Phosphorylation
K590 Ubiquitination
K605 Ubiquitination
K618 Ubiquitination
K669 Ubiquitination
K709 Ubiquitination
K720 Ubiquitination
Y722 Phosphorylation P12931 (SRC)
S724 Phosphorylation
K729 Ubiquitination
K749 Ubiquitination
K759 Ubiquitination
K795 Ubiquitination
K860 Ubiquitination
K877 Ubiquitination
S930 Phosphorylation
Y936 Phosphorylation
S937 Phosphorylation
K946 Acetylation
K951 Ubiquitination
K964 Ubiquitination
T967 Phosphorylation P53350 (PLK1)
K989 Ubiquitination
K995 Ubiquitination
K997 Ubiquitination
K1007 Ubiquitination
K1017 Ubiquitination
K1022 Ubiquitination
K1071 Ubiquitination
T1078 Phosphorylation
K1083 Ubiquitination
K1086 Ubiquitination
S1099 Phosphorylation P53350 (PLK1)
S1114 Phosphorylation
S1121 Phosphorylation
S1132 Phosphorylation
S1133 Phosphorylation P53350 (PLK1)
S1134 Phosphorylation
S1137 Phosphorylation
T1165 Phosphorylation
K1181 Ubiquitination
S1187 Phosphorylation
S1194 Phosphorylation
Y1197 Phosphorylation
T1212 Phosphorylation
K1222 Ubiquitination
Y1232 Phosphorylation
S1238 Phosphorylation
K1252 Ubiquitination
K1316 Ubiquitination
Y1318 Phosphorylation
Y1319 Phosphorylation
T1334 Phosphorylation
K1339 Ubiquitination
K1350 Acetylation
K1351 Acetylation
K1351 Ubiquitination
S1361 Phosphorylation
S1362 Phosphorylation
T1365 Phosphorylation
S1366 Phosphorylation
K1368 Methylation
K1368 Ubiquitination
S1374 Phosphorylation P53350 (PLK1)
S1379 Phosphorylation
S1388 Phosphorylation

翻译修饰 - O75116 作为激酶

Substrate Site Source
O14974 (PPP1R12A) T443 Uniprot
O14974 (PPP1R12A) T696 Uniprot
O14974 (PPP1R12A) S852 Uniprot
O14974-1 (PPP1R12A) T853 Uniprot
O75116 (ROCK2) T414 Uniprot
P05067-4 (APP) T654 Uniprot
P08670 (VIM) S39 Uniprot
P08670 (VIM) S72 Uniprot
P15311 (EZR) T567 Uniprot
P19634 (SLC9A1) T653 Uniprot
P24844 (MYL9) S20 Uniprot
P26038 (MSN) T558 Uniprot
P29474 (NOS3) T495 Uniprot
P35241 (RDX) T564 Uniprot
P35568 (IRS1) S636 Uniprot
P35568 (IRS1) S974 Uniprot
P35611 (ADD1) T445 Uniprot
P35611 (ADD1) T480 Uniprot
P51911 (CNN1) T170 Uniprot
P51911 (CNN1) S175 Uniprot
P51911 (CNN1) T180 Uniprot
P51911 (CNN1) T184 Uniprot
P51911 (CNN1) T259 Uniprot
P53671 (LIMK2) T505 Uniprot
P56817 (BACE1) S498 Uniprot
P68104 (EEF1A1) T432 Uniprot
Q15306 (IRF4) S447 Uniprot
Q15306 (IRF4) S448 Uniprot
Q15417 (CNN3) S293 Uniprot
Q16555 (DPYSL2) T555 Uniprot
Q92673 (SORL1) S2206 Uniprot
Q96A00 (PPP1R14A) T38 Uniprot
Q96C90 (PPP1R14B) T57 Uniprot
Q99962 (SH3GL2) T14 Uniprot

研究背景

功能:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

翻译修饰:

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.

Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

细胞定位:

Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the brain (at protein level).

亚基结构:

Homodimer (By similarity). Interacts with IRS1 (By similarity). Interacts with RAF1 (By similarity). Interacts with RHOA (activated by GTP), RHOB and RHOC (By similarity). Interacts with PPP1R12A. Interacts with EP300. Interacts with CHORDC1. Interacts with BRCA2. Interacts with NPM1; this interaction enhances ROCK2 activity. Interacts with SORL1.

蛋白家族:

An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). Baicalin Attenuates Panton-Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells. International journal of molecular sciences, 2023 (PubMed: 37833969) [IF=5.6]

Application: WB    Species: bovine    Sample: BMECs

Figure 3. Effects of rPVL on the regulation of RhoA/ROCK/LIMK/Cofilin and PI3K/AKT/GSK-3β signaling pathways and phosphorylation of cofilin and tau hyperphosphorylation in the rPVL-treated BMECs. Representative immunoblot bands for RhoA, p-ROCK2(Tyr722), ROCK2, p-LIMK1/2(Thr508/Thr505), p-cofilin (Ser3), and cofilin (A); p-PI3K (Tyr458/Tyr199), PI3K, p-AKT(Ser473), AKT, GSK-3β(Ser9), GSK-3β, p-tau (Ser396), and tau (B); GAPDH was used as a control. rPVL was used at a 100 ng/mL concentration. Data are expressed as mean ± standard deviation of three independent experiments. * 0.01 < p < 0.05, ** p < 0.01 (one-way ANOVA with Dunnett’s multiple comparison tests), ns: not significant.

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