产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
c Jun kinase 3; c-Jun N-terminal kinase 3; cJun N terminal kinase 3; FLJ12099; FLJ33785; JNK3 alpha protein kinase; JNK3; JNK3A; MAP kinase 10; MAP kinase; MAP kinase p49 3F12; MAPK 10; Mapk10; MGC50974; mitogen activated protein kinase 10; Mitogen-activated protein kinase 10; MK10_HUMAN; p493F12; p54bSAPK; PRKM10; protein kinase mitogen activated 10; SAPK1b; Stress activated protein kinase 1b; stress activated protein kinase beta; Stress activated protein kinase JNK3; Stress-activated protein kinase JNK3;
抗原和靶标
Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
- P53779 MK10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLHFLYYCSEPTLDVKIAFCQGFDKQVDVSYIAKHYNMSKSKVDNQFYSVEVGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLEASAGPLGCCR
翻译修饰 - P53779 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K94 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T131 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S182 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| T221 | Phosphorylation | O14733 (MAP2K7) | Uniprot |
| Y223 | Phosphorylation | P45985 (MAP2K4) | Uniprot |
| T226 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| K391 | Ubiquitination | Uniprot |
翻译修饰 - P53779 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O43521-2 (BCL2L11) | S58 | Uniprot |
| O43521-1 (BCL2L11) | S59 | Uniprot |
| O43521-1 (BCL2L11) | S69 | Uniprot |
| O43521-1 (BCL2L11) | S77 | Uniprot |
| O43521 (BCL2L11) | T116 | Uniprot |
| O60282 (KIF5C) | S176 | Uniprot |
| O95644 (NFATC1) | S172 | Uniprot |
| P01106 (MYC) | T58 | Uniprot |
| P01106 (MYC) | S62 | Uniprot |
| P01106 (MYC) | S71 | Uniprot |
| P04150 (NR3C1) | S226 | Uniprot |
| P04637 (TP53) | S37 | Uniprot |
| P05067-4 (APP) | T668 | Uniprot |
| P05067 (APP) | T743 | Uniprot |
| P05412 (JUN) | S63 | Uniprot |
| P05412 (JUN) | S73 | Uniprot |
| P10415 (BCL2) | T56 | Uniprot |
| P10415 (BCL2) | S70 | Uniprot |
| P10415 (BCL2) | T74 | Uniprot |
| P10415 (BCL2) | S87 | Uniprot |
| P10636 (MAPT) | T498 | Uniprot |
| P10636 (MAPT) | S516 | Uniprot |
| P10636 (MAPT) | S519 | Uniprot |
| P10636 (MAPT) | T522 | Uniprot |
| P10636 (MAPT) | T529 | Uniprot |
| P10636 (MAPT) | T534 | Uniprot |
| P10636 (MAPT) | S713 | Uniprot |
| P10636 (MAPT) | S721 | Uniprot |
| P10636 (MAPT) | S739 | Uniprot |
| P16949 (STMN1) | S25 | Uniprot |
| P16949 (STMN1) | S38 | Uniprot |
| P29353 (SHC1) | S36 | Uniprot |
| P30307 (CDC25C) | S168 | Uniprot |
| P31947 (SFN) | S186 | Uniprot |
| P54259 (ATN1) | S739 | Uniprot |
| P55957 (BID) | T59 | Uniprot |
| P61978-1 (HNRNPK) | S216 | Uniprot |
| P61978-1 (HNRNPK) | S284 | Uniprot |
| P61978-1 (HNRNPK) | S353 | Uniprot |
| P63104 (YWHAZ) | S184 | Uniprot |
| Q06481 (APLP2) | T736 | Uniprot |
| Q07820 (MCL1) | S121 | Uniprot |
| Q07820 (MCL1) | T163 | Uniprot |
| Q16600 (ZNF239) | S38 | Uniprot |
| Q16600 (ZNF239) | S129 | Uniprot |
| Q5JR12 (PPM1J) | S93 | Uniprot |
| Q93045 (STMN2) | S62 | Uniprot |
| Q93045 (STMN2) | S73 | Uniprot |
| Q9UPT6 (MAPK8IP3) | T265 | Uniprot |
| Q9UPT6 (MAPK8IP3) | T275 | Uniprot |
| Q9UPT6-1 (MAPK8IP3) | T286 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1.
Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
Palmitoylation regulates subcellular location and axonal development.
Cytoplasm. Membrane>Lipid-anchor. Nucleus. Mitochondrion.
Note: Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity).
Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Interacts with MAPKBP1 (By similarity). Interacts with MAPK8IP1/JIP-1 and MAPK8IP3/JIP-3/JSAP1 (By similarity). Interacts with SPAG9/MAPK8IP4/JIP4. Interacts with HDAC9. Interacts with ARRB2; the interaction enhances MAPK10 activation by MAP3K5. Interacts with SARM1 (By similarity). Interacts with JUND; interaction is inhibited in the presence of MEN1.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
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