产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; Osteosarcoma; p105-Rb; P105RB; PP105; pp110; PPP1R130; pRb; Prepro retinoblastoma associated protein; Protein phosphatase 1 regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB_HUMAN; RB1; RB1 gene; Retinoblastoma 1; Retinoblastoma suspectibility protein; Retinoblastoma-associated protein;
抗原和靶标
Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) (PubMed:20940255).
- P06400 RB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK
翻译修饰 - P06400 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| T5 | Phosphorylation | P11802 (CDK4) | Uniprot |
| S37 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | P24941 (CDK2) | Uniprot |
| Y239 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) , P05771 (PRKCB) , P06493 (CDK1) | Uniprot |
| T252 | Phosphorylation | P06493 (CDK1) , P05771 (PRKCB) , P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| K265 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| K359 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| T373 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| K420 | Ubiquitination | Uniprot | |
| K427 | Acetylation | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| Y454 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K548 | Acetylation | Uniprot | |
| S567 | Phosphorylation | Q16539 (MAPK14) , P24941 (CDK2) | Uniprot |
| K574 | Ubiquitination | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| T601 | Phosphorylation | Uniprot | |
| Y606 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| S612 | Phosphorylation | O96017 (CHEK2) , O14757 (CHEK1) , Q00534 (CDK6) , P11802 (CDK4) , P24941 (CDK2) | Uniprot |
| S618 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| T625 | Phosphorylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K720 | Sumoylation | Uniprot | |
| Y771 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| T774 | Phosphorylation | Uniprot | |
| R775 | Methylation | Uniprot | |
| T778 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | P11802 (CDK4) , P30279 (CCND2) , P17612 (PRKACA) , Q00534 (CDK6) , Q96GD4 (AURKB) , P05771 (PRKCB) | Uniprot |
| R787 | Methylation | Uniprot | |
| S788 | Phosphorylation | Q00534 (CDK6) , P11802 (CDK4) | Uniprot |
| Y790 | Phosphorylation | Uniprot | |
| K791 | Ubiquitination | Uniprot | |
| S794 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | P05771 (PRKCB) , P11802 (CDK4) , P50750 (CDK9) , Q00534 (CDK6) , Q00535 (CDK5) | Uniprot |
| R798 | Methylation | Uniprot | |
| Y805 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S807 | Phosphorylation | P24941 (CDK2) , P50750 (CDK9) , Q00534 (CDK6) , P11802 (CDK4) , Q00526 (CDK3) , P06493 (CDK1) , Q00535 (CDK5) | Uniprot |
| K810 | Methylation | Uniprot | |
| K810 | Ubiquitination | Uniprot | |
| S811 | Phosphorylation | Q00534 (CDK6) , P06493 (CDK1) , P24941 (CDK2) , P11802 (CDK4) , Q00526 (CDK3) , P50750 (CDK9) , Q00535 (CDK5) , Q13131 (PRKAA1) | Uniprot |
| Y813 | Phosphorylation | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| S816 | Phosphorylation | Uniprot | |
| T821 | Phosphorylation | P24941 (CDK2) , Q00534 (CDK6) , P05771 (PRKCB) | Uniprot |
| T823 | Phosphorylation | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| T826 | Phosphorylation | Q00534 (CDK6) , P11802 (CDK4) , P24941 (CDK2) | Uniprot |
| S829 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | Uniprot | |
| S838 | Phosphorylation | Uniprot | |
| T841 | Phosphorylation | Uniprot | |
| S842 | Phosphorylation | Uniprot | |
| K844 | Ubiquitination | Uniprot | |
| K847 | Ubiquitination | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| R857 | Methylation | Uniprot | |
| K860 | Methylation | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| S862 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| K870 | Ubiquitination | Uniprot | |
| K873 | Acetylation | Uniprot | |
| K874 | Acetylation | Uniprot | |
| S882 | Phosphorylation | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| K896 | Acetylation | Uniprot | |
| K896 | Ubiquitination | Uniprot | |
| K900 | Ubiquitination | Uniprot | |
| T905 | Phosphorylation | Uniprot | |
| S919 | Phosphorylation | Uniprot | |
| K925 | Acetylation | Uniprot |
研究背景
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).
(Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).
N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
Acetylated during keratinocyte differentiation. Acetylation at Lys-873 and Lys-874 regulates subcellular localization. Can be deacetylated by SIRT1.
Nucleus.
Note: During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization.
Expressed in the retina. Expressed in foreskin keratinocytes (at protein level).
Interacts with ATAD5. Interacts with PRMT2, CDK1 and CDK2 (By similarity). The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. Interacts with heterodimeric E2F/DP transcription factor complexes containing TFDP1 and either E2F1, E2F3, E2F4 or E2F5, or TFDP2 and E2F4. The unphosphorylated form interacts with EID1, ARID3B, KDM5A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with SNW1, AATF, DNMT1, LIN9, LMNA, KMT5B, KMT5C, PELP1, UHRF2 and TMPO-alpha. May interact with NDC80. Interacts with GRIP1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1 and LIMD1. Interacts with SMARCA4/BRG1 AND HDAC1 (By similarity). Interacts with PSMA3 and USP4. Interacts (when methylated at Lys-860) with L3MBTL1. Interacts with CHEK2; phosphorylates RB1. Interacts with CEBPA. P-TEFB complex interacts with RB1; promotes phosphorylation of RB1. Interacts with RBBP9; the interaction disrupts RB1 binding to E2F1 (By similarity). Interacts with KAT2B/PCAF and EP300/P300 (By similarity).
(Microbial infection) Interacts with adenovirus E1A protein.
(Microbial infection) Interacts with HPV E7 protein.
(Microbial infection) Interacts with SV40 large T antigen.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL123.
(Microbial infection) Interacts with molluscum contagiosum virus protein MC007.
The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
Belongs to the retinoblastoma protein (RB) family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.