产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
DKFZp781B1423; Double minute 4; Double minute 4 human homolog of p53 binding protein; Double minute 4 protein; HDMX; MDM 4; Mdm2 like p53 binding protein; Mdm2-like p53-binding protein; MDM4; Mdm4 p53 binding protein homolog mouse; Mdm4 protein; MDM4 related protein 1; Mdm4 transformed 3T3 cell double minute 4; Mdm4 transformed 3T3 cell double minute 4 p53 binding protein; Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse; MDM4_HUMAN; Mdmx protein; MGC132766; Mouse double minute 4 homolog; Mouse double minute 4 human homolog of p53 binding protein; MRP 1; MRP1; p53 binding protein; p53 BINDING PROTEIN MDM4; p53-binding protein Mdm4; Protein Mdm4; Protein Mdmx;
抗原和靶标
- O15151 MDM4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA
翻译修饰 - O15151 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S15 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K93 | Ubiquitination | Uniprot | |
| S96 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y99 | Phosphorylation | P00519 (ABL1) | Uniprot |
| T119 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K254 | Sumoylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S314 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S342 | Phosphorylation | P54646 (PRKAA2) , Q13315 (ATM) , O96017 (CHEK2) , O14757 (CHEK1) | Uniprot |
| T365 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | P31749 (AKT1) , Q13315 (ATM) , O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| K374 | Ubiquitination | Uniprot | |
| K379 | Sumoylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S403 | Phosphorylation | Q13315 (ATM) | Uniprot |
研究背景
Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent.
Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein.
Nucleus.
Expressed in all tissues tested with high levels in thymus.
Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with USP2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53.
Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
Belongs to the MDM2/MDM4 family.
研究领域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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