产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
KPCB_HUMAN; PKC beta; PKC-B; PKC-beta; PKCB; Prkcb; PRKCB I; PRKCB II; PRKCB1; PRKCB2; Protein kinase C beta 1; Protein kinase C beta 2; Protein kinase C beta; Protein kinase C beta type; protein kinase C, beta 1 polypeptide;
抗原和靶标
- P05771 KPCB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV
翻译修饰 - P05771 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| T314 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| T497 | Phosphorylation | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| T500 | Phosphorylation | P53004 (BLVRA) , O15530 (PDPK1) | Uniprot |
| T504 | Phosphorylation | Uniprot | |
| Y507 | Phosphorylation | Uniprot | |
| Y515 | Phosphorylation | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| K595 | Ubiquitination | Uniprot | |
| T642 | Phosphorylation | P05771 (PRKCB) | Uniprot |
| T644 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | P05771 (PRKCB) | Uniprot |
| Y662 | Phosphorylation | P43405 (SYK) | Uniprot |
翻译修饰 - P05771 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O15350 (TP73) | S388 | Uniprot |
| O15519 (CFLAR) | S193 | Uniprot |
| O95786 (DDX58) | S8 | Uniprot |
| O95786 (DDX58) | T170 | Uniprot |
| P04004 (VTN) | S381 | Uniprot |
| P04049 (RAF1) | S497 | Uniprot |
| P04049 (RAF1) | S619 | Uniprot |
| P04083 (ANXA1) | S27 | Uniprot |
| P04083 (ANXA1) | S28 | Uniprot |
| P05107 (ITGB2) | S745 | Uniprot |
| P05107 (ITGB2) | T758 | Uniprot |
| P05771-2 (PRKCB) | S16 | Uniprot |
| P05771-2 (PRKCB) | T17 | Uniprot |
| P05771-1 (PRKCB) | T635 | Uniprot |
| P05771 (PRKCB) | T642 | Uniprot |
| P05771-2 (PRKCB) | S660 | Uniprot |
| P05771 (PRKCB) | S661 | Uniprot |
| P06127 (CD5) | T434 | Uniprot |
| P06127 (CD5) | T436 | Uniprot |
| P06400 (RB1) | S249 | Uniprot |
| P06400 (RB1) | T252 | Uniprot |
| P06400 (RB1) | S780 | Uniprot |
| P06400 (RB1) | S795 | Uniprot |
| P06400 (RB1) | T821 | Uniprot |
| P06730-1 (EIF4E) | S209 | Uniprot |
| P07355-1 (ANXA2) | S2 | Uniprot |
| P07355-2 (ANXA2) | S20 | Uniprot |
| P07355 (ANXA2) | S26 | Uniprot |
| P10636-8 (MAPT) | S258 | Uniprot |
| P10636-8 (MAPT) | S293 | Uniprot |
| P10636-8 (MAPT) | S324 | Uniprot |
| P10636-8 (MAPT) | S352 | Uniprot |
| P11171-4 (EPB41) | S312 | Uniprot |
| P11388 (TOP2A) | S29 | Uniprot |
| P11473 (VDR) | S51 | Uniprot |
| P14598 (NCF1) | S303 | Uniprot |
| P14598 (NCF1) | S304 | Uniprot |
| P14598 (NCF1) | S315 | Uniprot |
| P14598 (NCF1) | S320 | Uniprot |
| P14598 (NCF1) | S328 | Uniprot |
| P14598 (NCF1) | S359 | Uniprot |
| P14598 (NCF1) | S370 | Uniprot |
| P14598 (NCF1) | S379 | Uniprot |
| P14679 (TYR) | S523 | Uniprot |
| P14679 (TYR) | S527 | Uniprot |
| P15336 (ATF2) | S121 | Uniprot |
| P16949 (STMN1) | S25 | Uniprot |
| P16949 (STMN1) | S38 | Uniprot |
| P17302 (GJA1) | S368 | Uniprot |
| P17677-1 (GAP43) | S41 | Uniprot |
| P19429 (TNNI3) | S42 | Uniprot |
| P19429 (TNNI3) | S44 | Uniprot |
| P19429 (TNNI3) | T143 | Uniprot |
| P19484 (TFEB) | S462 | Uniprot |
| P19484 (TFEB) | S463 | Uniprot |
| P19484 (TFEB) | S467 | Uniprot |
| P19484 (TFEB) | S469 | Uniprot |
| P20700 (LMNB1) | S395 | Uniprot |
| P20700 (LMNB1) | S405 | Uniprot |
| P21730 (C5AR1) | S334 | Uniprot |
| P26010-1 (ITGB7) | T783 | Uniprot |
| P28329-3 (CHAT) | T255 | Uniprot |
| P28329-3 (CHAT) | S346 | Uniprot |
| P28329-3 (CHAT) | S347 | Uniprot |
| P28329 (CHAT) | T373 | Uniprot |
| P28329-3 (CHAT) | S440 | Uniprot |
| P28329 (CHAT) | S464 | Uniprot |
| P28329 (CHAT) | S465 | Uniprot |
| P28329-3 (CHAT) | S476 | Uniprot |
| P28329 (CHAT) | S558 | Uniprot |
| P28329 (CHAT) | S594 | Uniprot |
| P28799 (GRN) | S81 | Uniprot |
| P29353 (SHC1) | S36 | Uniprot |
| P29474 (NOS3) | T495 | Uniprot |
| P29966 (MARCKS) | S159 | Uniprot |
| P29966 (MARCKS) | S163 | Uniprot |
| P29966 (MARCKS) | S170 | Uniprot |
| P30086 (PEBP1) | S153 | Uniprot |
| P31749 (AKT1) | T308 | Uniprot |
| P31749 (AKT1) | S473 | Uniprot |
| P34741 (SDC2) | S187 | Uniprot |
| P34741 (SDC2) | S188 | Uniprot |
| P35548 (MSX2) | T135 | Uniprot |
| P35548 (MSX2) | T141 | Uniprot |
| P35568 (IRS1) | S323 | Uniprot |
| P35568 (IRS1) | S441 | Uniprot |
| P35637 (FUS) | S257 | Uniprot |
| P41180 (CASR) | T888 | Uniprot |
| P41594-2 (GRM5) | S840 | Uniprot |
| P43629 (KIR3DL1) | S415 | Uniprot |
| P48736 (PIK3CG) | S582 | Uniprot |
| P49768 (PSEN1) | S346 | Uniprot |
| P49840 (GSK3A) | S21 | Uniprot |
| P49841 (GSK3B) | S9 | Uniprot |
| P55042 (RRAD) | S214 | Uniprot |
| P55042 (RRAD) | S257 | Uniprot |
| P55042 (RRAD) | S290 | Uniprot |
| P55042 (RRAD) | S299 | Uniprot |
| P56537-2 (EIF6) | T215 | Uniprot |
| P56537-1 (EIF6) | T234 | Uniprot |
| P56537 (EIF6) | S235 | Uniprot |
| P61764-1 (STXBP1) | S306 | Uniprot |
| P61764-2 (STXBP1) | S313 | Uniprot |
| P62491 (RAB11A) | S177 | Uniprot |
| P98082 (DAB2) | S24 | Uniprot |
| Q01844 (EWSR1) | S266 | Uniprot |
| Q03721 (KCNC4) | S8 | Uniprot |
| Q03721 (KCNC4) | S9 | Uniprot |
| Q03721 (KCNC4) | S15 | Uniprot |
| Q03721 (KCNC4) | S21 | Uniprot |
| Q05639 (EEF1A2) | S53 | Uniprot |
| Q06124-2 (PTPN11) | S576 | Uniprot |
| Q06124 (PTPN11) | S580 | Uniprot |
| Q06124 (PTPN11) | S595 | Uniprot |
| Q06187 (BTK) | S180 | Uniprot |
| Q07869 (PPARA) | S179 | Uniprot |
| Q07869 (PPARA) | S230 | Uniprot |
| Q12906 (ILF3) | S647 | Uniprot |
| Q13224 (GRIN2B) | S1303 | Uniprot |
| Q13224 (GRIN2B) | S1323 | Uniprot |
| Q14847 (LASP1) | S146 | Uniprot |
| Q16236 (NFE2L2) | S40 | Uniprot |
| Q16625-1 (OCLN) | S340 | Uniprot |
| Q16625 (OCLN) | S490 | Uniprot |
| Q5JVS0-1 (HABP4) | T354 | Uniprot |
| Q5JVS0 (HABP4) | T375 | Uniprot |
| Q5T7P8 (SYT6) | T283 | Uniprot |
| Q5T7P8 (SYT6) | T417 | Uniprot |
| Q5T7P8 (SYT6) | T418 | Uniprot |
| Q8NER1 (TRPV1) | T705 | Uniprot |
| Q92686 (NRGN) | S36 | Uniprot |
| Q96D31 (ORAI1) | S27 | Uniprot |
| Q96D31 (ORAI1) | S30 | Uniprot |
| Q96PH1-4 (NOX5) | T494 | Uniprot |
| Q96PH1-4 (NOX5) | S498 | Uniprot |
| Q99418-2 (CYTH2) | S391 | Uniprot |
| Q99418-1 (CYTH2) | S392 | Uniprot |
| Q9BXL7 (CARD11) | S559 | Uniprot |
| Q9BXL7 (CARD11) | S644 | Uniprot |
| Q9BXL7 (CARD11) | S652 | Uniprot |
| Q9H1D0 (TRPV6) | S184 | Uniprot |
| Q9H1D0 (TRPV6) | T728 | Uniprot |
| Q9P2D0 (IBTK) | S1200 | Uniprot |
| Q9P2D0 (IBTK) | S1203 | Uniprot |
| Q9UBS0 (RPS6KB2) | S473 | Uniprot |
| Q9UBU3 (GHRL) | S41 | Uniprot |
| Q9UQ80-1 (PA2G4) | S363 | Uniprot |
| Q9UQ80-1 (PA2G4) | T366 | Uniprot |
| Q9Y4H2 (IRS2) | S306 | Uniprot |
| Q9Y4H2 (IRS2) | S679 | Uniprot |
研究背景
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).
Cytoplasm. Nucleus. Membrane>Peripheral membrane protein.
Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Substance dependence > Morphine addiction.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.
限制条款
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